Anti-BDNF antibody (ab27932)

Overview

  • Product nameAnti-BDNF antibodySee all BDNF primary antibodies ...
  • Description
    Chicken polyclonal to BDNF
  • SpecificityThis antibody does not cross react with other neurotrophic factors.
  • Tested applicationsELISA, WB, Neutralising, ICC, IHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab27932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA
WB
Neutralising
ICC
IHC-P
  • Application notesELISA: Use at a concentration of 1 µg/ml.
    ICC: Use at a concentration of 1 - 10 µg/ml; methanol fixed: 1 - 10 µg/ml.
    IHC-P: Use at an assay dependent dilution.
    Neut: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
    • Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
    • Involvement in diseaseCongenital central hypoventilation syndrome
    • Sequence similaritiesBelongs to the NGF-beta family.
    • Post-translational
      modifications
      The propeptide is N-glycosylated and glycosulfated.
      Converted into mature BDNF by plasmin (PLG).
    • Cellular localizationSecreted.
    • Target information above from: UniProt accession P23560 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • Abrineurin antibody
      • BDNF antibody
      • BDNF antibody
      • BDNF_HUMAN antibody
      • Brain Derived Neurotrophic Factor antibody
      • Brain-derived neurotrophic factor antibody
      • MGC34632 antibody
      • Neurotrophin antibody
      see all

    References for Anti-BDNF antibody (ab27932)

    ab27932 has not yet been referenced specifically in any publications.

    Product Wall

    Merci de votre intérêt pour nos anticorps.

    Voici une les anticorps anti BDNF déjà testés par nous en coupes congélées et sur le tissu du rat, pour lesquels nous avons soit un retour d'un utilisateur (Abreview) soit une référence.

    ...

    Read More

    Thank you for contacting Abcam.

    As you mentioned the level of homology between species is highly conserved and so we cannot guarantee that any of our BDNF antibodies would only recognize the human sequence. On our datasheets, if we only say f...

    Read More
    Abreviews
    Application Western blot
    Sample Chicken Tissue lysate - whole (brain)
    Loading amount 5.2 µg
    Specification brain
    Treatment 1ul 200* proteinase inhibitor
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Ms. Ying Jiang

    Verified customer

    Submitted Sep 22 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"