Overview

  • Product nameAnti-BDNF antibody
    See all BDNF primary antibodies
  • Description
    Rabbit polyclonal to BDNF
  • SpecificityThe antibody is specific to recombinant BDNF protein and when used in rat brains extracts detects a 28kDa proBDNF band and in some tissues will also recognise the mature BDNF homodimer. This antibody shows no cross-reactivity with NGF, NT3 and NT4/5 using Western blot analysis.
  • Tested applicationsWB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A peptide from the N-terminal region of mature mouse BDNF conjugated to a carrier protein to enhance immunological response.

  • Positive control
    • Rat brain This antibody gave a positive result when used in the following formaldehyde fixed cell line: SKNSH.
  • General notesThis antibody recognizes both the mature BDNF and unprocessed precursor (proBDNF). BDNF is only seen as a 14 kDa band when it is run under reducing conditions. When a native gel is used, the dimeric form of BDNF is seen as a 28 kDa band.

Properties

Applications

Our Abpromise guarantee covers the use of ab46176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
ELISA 1/10000.
ICC/IF 1/200.

Target

  • FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
  • Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
  • Involvement in diseaseCongenital central hypoventilation syndrome
  • Sequence similaritiesBelongs to the NGF-beta family.
  • Post-translational
    modifications
    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abrineurin antibody
    • BDNF antibody
    • BDNF antibody
    • BDNF_HUMAN antibody
    • Brain Derived Neurotrophic Factor antibody
    • Brain-derived neurotrophic factor antibody
    • MGC34632 antibody
    • Neurotrophin antibody
    see all

Anti-BDNF antibody images

  • ICC/IF image of ab46176 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46176 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-BDNF antibody (ab46176) at 1/1000 dilution

    Lane 1 : Rat Brain at 10 µl
    Lane 2 : negative control


    Predicted band size : 28 kDa
    Observed band size : 28 kDa

References for Anti-BDNF antibody (ab46176)

This product has been referenced in:
  • Vahdati Hassani F  et al. Antidepressant effects of crocin and its effects on transcript and protein levels of CREB, BDNF, and VGF in rat hippocampus. Daru 22:16 (2014). WB ; Rat . Read more (PubMed: 24401376) »
  • Hannah-Poquette C  et al. Modeling mania: Further validation for Black Swiss mice as model animals. Behav Brain Res 223:222-6 (2011). ICC/IF ; Mouse . Read more (PubMed: 21570428) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (4-15%gel)
Sample Human Cell lysate - whole cell (Lung)
Specification Lung
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 11 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted May 08 2013

Thank you very much for contacting us with your enquiry and for sending the information about your protocol and results.
Could you also tell me what kind of cell extracts you're working with? From what species and tissue/cell type?
Other user...

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Thank you for contacting us.

As we discussed on the phone, the 28 kDa band is consistent with the band observed for proBDNF. BDNF also forms homodimers, so the 42 kDa band is likely a dimer. You may be able to break down the dimer into monome...

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Thank you for contacting us.

I am very sorry to hear that you have been experiencing problems using this latest vial of ab46176.This product is covered by our Abpromise guarantee. If we cannot remedy this issue and this is a product that y...

Read More

Thank you for contacting Abcam.

This antibody recognizes both the mature BDNF and unprocessed precursor (proBDNF). BDNF is only seen as a 14 kDa band when it is run under reducing conditions. When a native gel is used, the dimeric form of BDNF...

Read More

Thank you for contacting Abcam.

This antibody recognizes both the mature BDNF and unprocessed precursor (proBDNF). BDNF is only seen as a 14 kDa band when it is run under reducing conditions. When a native gel is used, the dimeric form of BDNF...

Read More

Thank you very much for your interest in ab46176.

Unfortunately, we have not heard back from the customer in questions 4.

Therefore, I can offer a discount off a future purchase if you buy ab46176 andab28952now, test it insandwich ...

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Thank you for your enquiry.

For BDNF, I can suggest choosing the antibody that has been tested in IHC-P (paraffin embedded sections) or IHC-Fr (frozen sections). Whichever one of these applications you are using, I recommend to select an ant...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Brain)
Loading amount 20 µg
Specification Brain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Dec 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"