Anti-BDNF antibody (ab46176)
- Product nameAnti-BDNF antibodySee all BDNF primary antibodies ...
- DescriptionRabbit polyclonal to BDNF
- SpecificityThe antibody is specific to recombinant BDNF protein and when used in rat brains extracts detects a 28kDa proBDNF band and in some tissues will also recognise the mature BDNF homodimer. This antibody shows no cross-reactivity with NGF, NT3 and NT4/5 using Western blot analysis.
- Tested applicationsWB, ELISA, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Human
A peptide from the N-terminal region of mature mouse BDNF conjugated to a carrier protein to enhance immunological response.
- Positive control
- Rat brain This antibody gave a positive result when used in the following formaldehyde fixed cell line: SKNSH.
- General notesThis antibody recognizes both the mature BDNF and unprocessed precursor (proBDNF). BDNF is only seen as a 14 kDa band when it is run under reducing conditions. When a native gel is used, the dimeric form of BDNF is seen as a 28 kDa band.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: Whole serum
- PurityWhole antiserum
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab46176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
- FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
- Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
- Involvement in diseaseCongenital central hypoventilation syndrome
- Sequence similaritiesBelongs to the NGF-beta family.
modificationsThe propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG).
- Cellular localizationSecreted.
- Abrineurin antibody
- BDNF antibody
- BDNF antibody
- BDNF_HUMAN antibody
- Brain Derived Neurotrophic Factor antibody
- Brain-derived neurotrophic factor antibody
- MGC34632 antibody
- Neurotrophin antibody
Anti-BDNF antibody images
ICC/IF image of ab46176 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46176 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-BDNF antibody (ab46176) at 1/1000 dilution
Lane 1 : Rat Brain at 10 µl
Lane 2 : negative control
Predicted band size : 28 kDa
Observed band size : 28 kDa
References for Anti-BDNF antibody (ab46176)
This product has been referenced in:
- Hannah-Poquette C et al. Modeling mania: Further validation for Black Swiss mice as model animals. Behav Brain Res 223:222-6 (2011). ICC/IF ; Mouse . Read more (PubMed: 21570428) »
- Puerta E et al. Sildenafil protects against 3-nitropropionic acid neurotoxicity through the modulation of calpain, CREB, and BDNF. Neurobiol Dis 38:237-45 (2010). WB ; Rat . Read more (PubMed: 20109548) »