Overview
- Product nameAnti-BDNF antibodySee all BDNF primary antibodies ...
- DescriptionRabbit polyclonal to BDNF
- SpecificityThe antibody is specific to recombinant BDNF protein and when used in rat brains extracts detects a 28kDa proBDNF band and in some tissues will also recognise the mature BDNF homodimer. This antibody shows no cross-reactivity with NGF, NT3 and NT4/5 using Western blot analysis.
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
A peptide from the N-terminal region of mature mouse BDNF conjugated to a carrier protein to enhance immunological response.
- Positive controlRat brain
- General notesThis antibody recognizes both the mature BDNF and unprocessed precursor (proBDNF). BDNF is only seen as a 14 kDa band when it is run under reducing conditions. When a native gel is used, the dimeric form of BDNF is seen as a 28 kDa band.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: Whole serum - PurityWhole antiserum
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab46176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
| ELISA | ELISA: 1/10000. |
Target
- FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
- Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
- Involvement in diseaseCongenital central hypoventilation syndrome (CCHS) [MIM:209880]: Rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Note=The disease is caused by mutations affecting the gene represented in this entry.
- Sequence similaritiesBelongs to the NGF-beta family.
- Post-translational
modificationsThe propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG). - Cellular localizationSecreted.
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Database links
- Entrez Gene: 627 Human
- Entrez Gene: 12064 Mouse
- Entrez Gene: 24225 Rat
- Omim: 113505 Human
- SwissProt: P23560 Human
- SwissProt: P21237 Mouse
- SwissProt: P23363 Rat
- Unigene: 502182 Human
- Unigene: 1442 Mouse
- Unigene: 11266 Rat
see all
Target information above from: UniProt accession
P23560
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Abrineurin antibodyBDNF antibodyBDNF antibody
- BDNF_HUMAN antibodyBrain Derived Neurotrophic Factor antibodyBrain-derived neurotrophic factor antibodyMGC34632 antibodyNeurotrophin antibody
see all
Anti-BDNF antibody images
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Western blot - Anti-BDNF antibody (ab46176)All lanes : Anti-BDNF antibody (ab46176) at 1/1000 dilution
Lane 1 : Rat Brain at 10 µl
Lane 2 : negative control
Predicted band size : 28 kDa
Observed band size : 28 kDa
References for Anti-BDNF antibody (ab46176)
This product has been referenced in:
- Hannah-Poquette C et al. Modeling mania: Further validation for Black Swiss mice as model animals. Behav Brain Res 223:222-6 (2011). ICC/IF ; Mouse . Read more (PubMed: 21570428) »
- Puerta E et al. Sildenafil protects against 3-nitropropionic acid neurotoxicity through the modulation of calpain, CREB, and BDNF. Neurobiol Dis 38:237-45 (2010). WB ; Rat . Read more (PubMed: 20109548) »
