BDNF antibody (ab46176)

Product Name 

BDNF antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to BDNF

Immunogen 

A peptide from the N-terminal region of mature mouse BDNF conjugated to a carrier protein to enhance immunological response.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

The antibody is specific to recombinant BDNF protein and when used in rat brains extracts detects a 28kDa proBDNF band and in some tissues will also recognise the mature BDNF homodimer.
This antibody shows no cross-reactivity with NGF, NT3 and NT4/5 using Western blot analysis.

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/10000.
WB: 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Rat brain

Cellular localization 

Secreted

Research areas 

Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cardiovascular >> Atherosclerosis >> Diabetes associated
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease
Neuroscience >> Neurology process >> Growth and Development >> Neurotrophins

Relevance 

Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non neuronal cells. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients. In addition, functional studies showed that age-associated changes in BDNF-mediated pathways can enhance inflammation and increase myocardial injury after myocardial infarction in the aging heart.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: Whole serum

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Notes 

This antibody recognizes both the mature BDNF and unprocessed precursor (proBDNF). BDNF is only seen as a 14 kDa band when it is run under reducing conditions. When a native gel is used, the dimeric form of BDNF is seen as a 28 kDa band.

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