Overview

  • Product nameAnti-BDNF antibodySee all BDNF primary antibodies ...
  • Description
    Rabbit polyclonal to BDNF
  • SpecificityBy dot blots, less than 0.1% cross-reactivity against mouse NGF, recombinant human NT3 or neurotrophin 4/5. Cross reactivity: Known to react with BDNF from rat and human. Cross-reactivity with other species has not been determined yet.
  • Tested applicationsDot Blot, IHC-Fr, Neutralising, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • General notesThis antibody has proven particularly useful for western blot, immunohistochemistry and biological inhibition. For immunohistochemistry, cell bodies are clearly stained as well as some nerve terminals in the dorsal horn of the rat spinal cord. The antiserum does not stain the finest nerve terminals.

Properties

Applications

Our Abpromise guarantee covers the use of ab6200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot Blot
IHC-Fr
Neutralising
WB
  • Application notesImmunohistochemistry: 1/1,000
    Western Blot: 1/1,000
    Immunoblot: 1/1,000
    1 site ELISA: 1/1,000
    Biological Inhibition: 5- 10 ul/ gm body weight in vivo.
  • Target

    • FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
    • Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
    • Involvement in diseaseCongenital central hypoventilation syndrome
    • Sequence similaritiesBelongs to the NGF-beta family.
    • Post-translational
      modifications
      The propeptide is N-glycosylated and glycosulfated.
      Converted into mature BDNF by plasmin (PLG).
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Abrineurin antibody
      • BDNF antibody
      • BDNF antibody
      • BDNF_HUMAN antibody
      • Brain Derived Neurotrophic Factor antibody
      • Brain-derived neurotrophic factor antibody
      • MGC34632 antibody
      • MGC34632 antibody
      • Neurotrophin antibody
      see all

    References for Anti-BDNF antibody (ab6200)

    ab6200 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.
    The expected MW for BDNF is 28 kD.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Thank you for your enquiry. We are very sorry to hear that you are having problem with these two antibodies. According to the datasheets of these products, ab9793 and ab6200 have only been testes on human samples and we do not know if they recognize...

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    We apologize for this large difference in the volume of the final product. We are unsure the cause for the large volume lost in volume even after a QC to check the volume of each vial prior to delivery. Perhaps, the problem may be caused by the change ...

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    Super - glad to hear that ab6200 is now working! Thank you for letting me know.

    I am sorry to hear that you are having trouble getting our Rabbit polyclonal to human Brain Derived Neurotrophic Factor (BDNF) to work in WB. I have checked the datasheet and see that the antibody has been shown to cross react with rat BDNF and you are...

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    Yes, this antibody will recognise the rat molecule.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"