Anti-BDNF antibody (ab6201)

Immunohistochemistry - Free Floating - BDNF antibody (ab6201)

ab6201 BDNF antibody immunostaining in the ventral horn of the spinal cord. Protocol: free floating, PFA/picric acid perfusion fixed rat coronal sections (30 microns) were incubated overnight in ab6201 (1/500) followed by 1 night of postfixation). Immunofluorescence was visualised with TSA amplification.

Sophie Pezet, King`s college London, United Kingdom

Immunohistochemistry - Free Floating - BDNF antibody (ab6201)

ab6201 BDNF antibody immunostaining BDNF-containing hippocampal neurons. Protocol: PFA(only)-fixed free floating coronal rat brain sections (30 microns) were incubated for 3 days with ab6201 at 1/100 followed by direct immunofluorescence detection (alexa488; 1/1000). We recommend using an amplification IHC protocol following PFA/picric acid fixation of brain tissue for optimising BDNF detection using ab6201

Sophie Pezet, King`s college London, United Kingdom

Immunocytochemistry/ Immunofluorescence-BDNF antibody(ab6201)

ICC/IF image of ab6201 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6201, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Western blot - Anti-BDNF antibody (ab6201)

Anti-BDNF antibody (ab6201) at 1 µg/ml + BDNF protein (ab9794) at 0.1 µg

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Exposure time : 4 minutes