Anti-BDNF antibody (ab6201)

That sounds great. Please send the replacement vial to the same address as the one for the original vial. I will hopefully have better luck with this replacement. I will contact you again if I am having trouble with the replacement vial. Thanks again.

Best Regards,

ANSWER:

Thank you for confirming these details and for your cooperation. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1065154.

To check the status of the order please contact our Customer Service team and reference this number.

As said, please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for being very attentive to my response. Would it be possible toreceivea vial of the antibody of a different batch in a new purification but also receive a new vial of a different lot number (once it becomes available in stock) if the antibody of the different batch produces the same results as the last one and does not work? Please let me know.
Thanks,

ANSWER:

Thank you for your quick reply.

Yes, this is no problem. The replacement vial, of the same batch or not, is also covered by our Abpromise guarantee. Shall I proceed then and send the replacement vial of the new purification to the same address as the original vial?

I look forward hearing from you.

I have attached pictures that I have taken of 20 micron rat brain sections stained with abcam's anti-BDNF. They are viewed under 50X and 200X and are labeled as so. The pictures I have attached are those of 1 day incubation (1-200 and 1-1000 dilutions) and 2 day incubation (1-200 and 1-1000 dilutions). I also have 3 day incubation sections that I have taken but not have attached in this email. Let me know if you would like to see those as well.

I have also attached a couple of images of anti-BDNF staining done in the past that came with good results. These were done under the same conditions but the antibody was ordered from a different company. I have attached it so you could perhaps use it as a reference to how correct staining should look like.

Thanks,

ANSWER:

Thank you for taking time to provide all these images and for contacting us. I am sorry to hear and see now thatthis antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

As said on the phone, and confirmed now by viewing the images,I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

As promised, I inquired about the exact origin of the different batch we have in stock. I confirm now that this batch is form the same rabbits as you have obtained, however from a different purification. Please let me know whether you would like to receive a vial of the new purification or whether you prefer another antibody.

Thank you for your cooperation. I look forward to hearing from you.

Phone call: Customer uses this antibody on rat brain perfusion fixed samples. There is no specific staining, just backgound.
Secondary antibody is good, permeabilisation done with Triton, other antibodies work, blocking with 10% NGS, incubated primary antibody between 1 to 3 days at 4 degrees, concentrations between 1/200 to 1/1000.
Will send image.

ANSWER:

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the phone, I have requested further information on the batches we have in stock. Please do send me also the image with the results of the staining, this will help us to investigate this case internally and initiate additional testing where necessary.

I look forward to receiving your reply.

I want to work with the next primary antibodies for ICC/IF,I wonder if I can use them on rats:



Anti-CD4 antibody [10C12] (ab25804)

Anti-T Cell Receptor V gamma 3 antibody [536] (ab21029)

Anti-BDNF antibody (ab6201)

ANSWER:

Thank you for contacting Abcam and for your interest in our products.

Regarding the CD4 antibody ab25804, based on a BLAST of the immunogen, there is no homology with rat, therefore I would not recommend this antibody to use with rat samples. As an alternative, I would like to recommend ab33775. Although it is not tested for ICC/IF and therefore not covered by our Abpromise, I am happy to offer a testing discount in which you purchase the antibody, test it in ICC/IF and submit your results in the form of an Abreview. Once the review has been submitted, the code I issue can be redeemed for a free primary antibody on a future purchase. Please see the terms and conditions for additional information: www.abcam.com/collaborationdiscount.

Regarding ab21029 (T cell Receptor V gamma 3), this antibody is not validated for rat and the epitope has not been mapped. Therefore, we do not know if the antibody will react, but there is 84% overall homology between the mouse and rat proteins so there is a chance it might work. The same testing discount above would apply.

Finally, ab6201 (BDNF), is tested and guaranteed to work with rat samples in ICC/IF.

I hope this information is helpful. If you are interested in learning more about the testing discount, or if you would like to test ab21029 and ab33775, please reply to this email and I will issue the discount codes. Please do not hesitate to contact me if you have any additional questions.