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Products:Neuroscience >> Neurology process >> Growth and Development >> Neurotrophins
Overview
Product name
Anti-BDNF antibody
See all BDNF products (22) ...
Description
Rabbit polyclonal to BDNF
Specificity
By dot blots, less than 0.1% cross-reactivity against mouse NGF, recombinant human NT3 or neurotrophin 4/5.
Tested applications
Dot Blot, ICC/IF, ELISA, Neutralising, WB, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Recombinant full length protein (Human).
Positive control
IHC-Fr: rat dorsal root ganglion (using a ganglion from an animal perfused with 2% (2% not the usual 4%) formaldehyde, with the addition of 15% picric acid, will reveal BDNF in many small diameter neurons. WB: dorsal root ganglia is recommended as the best positive control, but if not available, then both brain or spinal cord extracts can be used.
General notes
This antibody has proven particularly useful for western blot, immunohistochemistry and biological inhibition. For immunohistochemistry, cell bodies are clearly stained as well as some nerve terminals in the dorsal horn of the rat spinal cord. The antiserum does not stain the finest nerve terminals.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10mM PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Light chain type
unknown
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cardiovascular >> Atherosclerosis >> Diabetes associated
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease
Neuroscience >> Neurology process >> Growth and Development >> Neurotrophins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use a concentration of 1 - 5 µg/ml.
ICC/IF: Use a concentration of 5 µg/ml
ELISA: Use a concentration of 0.1 µg/ml
Neut: Use a concentration of 2 - 10 µg/ml.
WB: Use a concentration of 1 - 5 µg/ml.
IHC-FoFr: Use a concentration of 1 - 5 µg/ml.(PubMed: 18971468Using ABC or tyramide amplification may be required to enhance the signal.)
Target
Function
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Tissue specificity
Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
Involvement in disease
Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
Sequence similarities
Belongs to the NGF-beta family.
Post-translational
modifications
The propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG).
Cellular localization
Secreted.
Target information above from: UniProt accessionP23560
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Abrineurin antibody
- BDNF antibody
- BDNF antibody
see all
Database links
- Entrez Gene: 627 Human
- Entrez Gene: 12064 Mouse
- Entrez Gene: 24225 Rat
- Omim: 113505 Human
- SwissProt: P23560 Human
- SwissProt: P21237 Mouse
- SwissProt: P23363 Rat
- Unigene: 502182 Human
see all
Anti-BDNF antibody images:
Immunohistochemistry - Free Floating - BDNF antibody (ab6201)
ab6201 BDNF antibody immunostaining in the ventral horn of the spinal cord. Protocol: free floating, PFA/picric acid perfusion fixed rat coronal sections (30 microns) were incubated overnight in ab6201 (1/500) followed by 1 night of postfixation). Immunofluorescence was visualised with TSA amplification.
Sophie Pezet, King`s college London, United Kingdom
Immunohistochemistry - Free Floating - BDNF antibody (ab6201)
ab6201 BDNF antibody immunostaining BDNF-containing hippocampal neurons. Protocol: PFA(only)-fixed free floating coronal rat brain sections (30 microns) were incubated for 3 days with ab6201 at 1/100 followed by direct immunofluorescence detection (alexa488; 1/1000). We recommend using an amplification IHC protocol following PFA/picric acid fixation of brain tissue for optimising BDNF detection using ab6201
Sophie Pezet, King`s college London, United Kingdom
Immunocytochemistry/ Immunofluorescence-BDNF antibody(ab6201)
ICC/IF image of ab6201 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6201, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-BDNF antibody (ab6201)
Anti-BDNF antibody (ab6201) at 1 µg/ml + BDNF protein (ab9794) at 0.1 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutes
References for Anti-BDNF antibody (ab6201)
This product has been referenced in:
- Côté MPet al. Activity-dependent increase in neurotrophic factors is associated with an enhanced modulation of spinal reflexes after spinal cord injury. J Neurotrauma 28:299-309 (2011). WB; Rat.Read more (PubMed: 21083432) »
- Mashayekhi Fet al. Expression of insulin-like growth factor-1 and insulin-like growth factor binding proteins in the serum and cerebrospinal fluid of patients with Parkinson's disease. J Clin Neurosci 17:623-7 (2010). WB; Human.Read more (PubMed: 20189394) »
See all 6 publications for this product
Publishing research using ab6201? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"