Overview

  • Product nameAnti-BDNF antibody
    See all BDNF primary antibodies
  • Description
    Rabbit polyclonal to BDNF
  • SpecificityBy dot blots, less than 0.1% cross-reactivity against mouse NGF, recombinant human NT3 or NT4. Regarding use with brain lysates in WB: BDNF is very weakly expressed in whole brain (orders of magnitude lower), and extremely difficult to detect in BRAIN lysates. Thus, it cannot be compared to structural proteins or more common targets. It is recommended to use a positive control or spiked lysate control to optimize WB conditions.
  • Tested applicationsDot Blot, ICC/IF, ELISA, Neutralising, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Human, Pig
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • Purchase matching WB positive control:Active human BDNF full length protein
    • IHC-Fr: rat dorsal root ganglion (using a ganglion from an animal perfused with 2% (2% not the usual 4%) formaldehyde, with the addition of 15% picric acid, will reveal BDNF in many small diameter neurons. WB: dorsal root ganglia is recommended as the best positive control, but if not available, then both brain or spinal cord extracts can be used.
  • General notesThis antibody has proven particularly useful for western blot, immunohistochemistry and biological inhibition. For immunohistochemistry, cell bodies are clearly stained as well as some nerve terminals in the dorsal horn of the rat spinal cord. The antiserum does not stain the finest nerve terminals.

Properties

Applications

Our Abpromise guarantee covers the use of ab6201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot Blot Use a concentration of 1 - 10 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
ELISA Use a concentration of 0.1 µg/ml.
Neutralising Use a concentration of 2 - 10 µg/ml.
WB Use a concentration of 1 - 10 µg/ml.

WB analysis of BDNF can give multiple bands, especially when using tissue lysates, because of many BDNF binding proteins that can form SDS-stable complexes even after boiling. Even in reduced samples, BDNF forms easily dimers and multimers, thus six or more bands can be present (most prominent: 28kDa dimer, monomer: ~14kDa). ab6201 will react also with BDNF-proforms (32-45kDa). It might be advisable to use acidified samples for WB and perform an IP first.

IHC-FoFr Use a concentration of 1 - 5 µg/ml. PubMed: 18971468Using ABC or tyramide amplification may be required to enhance the signal.

Target

  • FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
  • Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
  • Involvement in diseaseCongenital central hypoventilation syndrome
  • Sequence similaritiesBelongs to the NGF-beta family.
  • Post-translational
    modifications
    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abrineurin antibody
    • BDNF antibody
    • BDNF antibody
    • BDNF_HUMAN antibody
    • Brain Derived Neurotrophic Factor antibody
    • Brain-derived neurotrophic factor antibody
    • MGC34632 antibody
    • MGC34632 antibody
    • Neurotrophin antibody
    see all

Anti-BDNF antibody images

  • All lanes : Anti-BDNF antibody (ab6201)

    Lane 1 : Purified rHBNDF at 0.05 µg
    Lane 2 : Mouse brian lysate at 15 µg


    Observed band size : 14,28 kDa (why is the actual band size different from the predicted?)
  • ab6201 BDNF antibody immunostaining in the ventral horn of the spinal cord. Protocol: free floating, PFA/picric acid perfusion fixed rat coronal sections (30 microns) were incubated overnight in ab6201 (1/500) followed by 1 night of postfixation). Immunofluorescence was visualised with TSA amplification.
  • ab6201 BDNF antibody immunostaining BDNF-containing hippocampal neurons. Protocol: PFA(only)-fixed free floating coronal rat brain sections (30 microns) were incubated for 3 days with ab6201 at 1/100 followed by direct immunofluorescence detection (alexa488; 1/1000). We recommend using an amplification IHC protocol following PFA/picric acid fixation of brain tissue for optimising BDNF detection using ab6201
  • ICC/IF image of ab6201 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6201, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-BDNF antibody (ab6201)

This product has been referenced in:
  • Chen L  et al. Ginseng Total Saponins Reverse Corticosterone-Induced Changes in Depression-Like Behavior and Hippocampal Plasticity-Related Proteins by Interfering with GSK-3 ß -CREB Signaling Pathway. Evid Based Complement Alternat Med 2014:506735 (2014). WB . Read more (PubMed: 24523822) »
  • Tapocik JD  et al. microRNA-206 in rat medial prefrontal cortex regulates BDNF expression and alcohol drinking. J Neurosci 34:4581-8 (2014). Rat . Read more (PubMed: 24672003) »

See all 14 Publications for this product

Product Wall

Thank you for contacting us.

All three products seem to be suitable for your application and are reacting with rat, although there are a few differences to be considered.

ab101748
This antibody positively tested for IHC on frozen ...

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Application Western blot
Sample Chicken Tissue lysate - whole (brain)
Loading amount 5.2 µg
Specification brain
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Ms. Ying Jiang

Verified customer

Submitted Aug 16 2012

Thank you very much for your call today and for your interest in these antibodies.

Since the antibodies ab19857 and ab72543havenot yetbeen tested inon perfused-fixed tissue (IHC-FoFr),I can offer discounts off a future purchase if you purcha...

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Thank you for confirming these details and for your cooperation. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order numbe...

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Thank you for your quick reply.

Yes, this is no problem. The replacement vial, of the same batch or not, is also covered by our Abpromise guarantee. Shall I proceed then and send the replacement vial of the new purification to the same addres...

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Thank you for taking time to provide all these images and for contacting us. I am sorry to hear and see now thatthis antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provid...

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Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the ...

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Thank you for contacting Abcam and for your interest in our products.

Regarding the CD4 antibody ab25804, based on a BLAST of the immunogen, there is no homology with rat, therefore I would not recommend this antibody to use with rat samples...

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Thank you for your enquiry. I am pleased to help answer your questions:

1. I would like to reassure you that both ab108383 and ab6201 are succesfully tested and therefore covered by the Abcam guarantee in ICC-IF. Therefore, any BDNF antibody...

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I apologize that this has happened again. Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products. We would be pleased to arrange a replacement product free of charge with a different lot. I a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"