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Recombinant full length protein (Human).
Our Abpromise guarantee covers the use of ab6201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot Blot||Use a concentration of 1 - 10 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|ELISA||Use a concentration of 0.1 µg/ml.|
|Neutralising||Use a concentration of 2 - 10 µg/ml.|
|WB||Use a concentration of 1 - 10 µg/ml.
WB analysis of BDNF can give multiple bands, especially when using tissue lysates, because of many BDNF binding proteins that can form SDS-stable complexes even after boiling. Even in reduced samples, BDNF forms easily dimers and multimers, thus six or more bands can be present (most prominent: 28kDa dimer, monomer: ~14kDa). ab6201 will react also with BDNF-proforms (32-45kDa). It might be advisable to use acidified samples for WB and perform an IP first.
|IHC-FoFr||Use a concentration of 1 - 5 µg/ml. PubMed: 18971468Using ABC or tyramide amplification may be required to enhance the signal.|
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