Overview

  • Product nameAnti-BDNF antibodySee all BDNF primary antibodies ...
  • Description
    Rabbit polyclonal to BDNF
  • SpecificityThis antibody appears to be specific for BDNF; it shows no cross-reactivity with other neurotrophic factors.
  • Tested applicationsIHC-FoFr, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide from human BDNF conjugated to an immunogenic carrier protein. The peptide is homologous in many other species including rat, mouse, zebra fish and xenopus. Peptide available as ab97400.

Properties

Applications

Our Abpromise guarantee covers the use of ab72439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 24510125
WB Use a concentration of 10 - 50 µg/ml. Predicted molecular weight: 28 kDa.Can be blocked with BDNF peptide (ab97400).
IHC-P Use a concentration of 10 - 50 µg/ml.

Antigen retrieval is recommended.

IHC-Fr Use a concentration of 10 - 50 µg/ml.

Target

  • FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
  • Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
  • Involvement in diseaseCongenital central hypoventilation syndrome
  • Sequence similaritiesBelongs to the NGF-beta family.
  • Post-translational
    modifications
    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abrineurin antibody
    • BDNF antibody
    • BDNF antibody
    • BDNF_HUMAN antibody
    • Brain Derived Neurotrophic Factor antibody
    • Brain-derived neurotrophic factor antibody
    • MGC34632 antibody
    • Neurotrophin antibody
    see all

Anti-BDNF antibody images

  • ab72439 staining BDNF in Mouse ovarian tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was blocked with 10% serum in 0.1% Triton X-100; antigen retrieval was by heat mediation in a citrate buffer (pH 6.0). Samples were incubated with primary antibody (1/100) overnight at 4°C. A HRP-conjugated Horse anti-rabbit IgG polyclonal (1/2000) was used as the secondary antibody.

  • Anti-BDNF antibody (ab72439) at 1/10 dilution + SK N SH (Human neuroblastoma) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.

References for Anti-BDNF antibody (ab72439)

This product has been referenced in:
  • Guo S  et al. IRF4 is a novel mediator for neuronal survival in ischaemic stroke. Cell Death Differ 21:888-903 (2014). WB, IHC-FoFr ; Mouse . Read more (PubMed: 24510125) »
  • Lambert KG  et al. Contingency-based emotional resilience: effort-based reward training and flexible coping lead to adaptive responses to uncertainty in male rats. Front Behav Neurosci 8:124 (2014). IHC-FrFl ; Rat . Read more (PubMed: 24808837) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Commercial Peroxidase blocking buffer as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Commercial High pH buffer
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative Formaldehyde
Username

Dr. Sarah Franklin

Verified customer

Submitted Aug 14 2014

Thank you for this additional information. One thing which may improve your signal is increasing the dilution even more. Our datasheet recommend 10-50 ug/ml, which is 1/100-1/20. I would try using 1/20 overnight at 4 degrees, since for this antibody yo...

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Thank you for kindly confirming these details. I am sorry these vials of antibody have not worked for this customer.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial each of ab6789, ab72439 and ...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion.

I appreciate the time the customer has spent on these experiments, and ...

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I am very pleased to hear you would like to accept our offer and test ab133195 and ab72439 in frozen chicken sections in IHC-Fr.Each code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abrev...

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Thank you for contacting us.
Your credit note ID for the refund is xxx.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, p...

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Thank you for your message and I appreciate the cooperation from the customer. It is regrettable that three antibodies have not worked for them.

I look forward to hearing from you with the results of the suggestions made. I hope these will h...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thank you for your reply, and I apologize for this inconvenience.

I certainly understand that ab9858 is not as useful to you as ab73680 would be. I can issue a credit to you.

The vial of ab8020 should be arriving shortly, please kee...

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Thank you again for your patience.

I apologize, but ab73680 is out of stock, and it may not be put back into stock for several months as recent lots have not passed quality control testing. Would you be interested in trying another vial of ab...

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