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A synthetic peptide from human BDNF conjugated to an immunogenic carrier protein. The peptide is homologous in many other species including rat, mouse, zebra fish and xenopus. Peptide available as ab97400.
Our Abpromise guarantee covers the use of ab72439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 24510125|
|WB||Use a concentration of 10 - 50 µg/ml. Predicted molecular weight: 28 kDa.Can be blocked with BDNF peptide (ab97400).|
|IHC-P||Use a concentration of 10 - 50 µg/ml.
Antigen retrieval is recommended.
|IHC-Fr||Use a concentration of 10 - 50 µg/ml.|
ab72439 staining BDNF in Mouse ovarian tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was blocked with 10% serum in 0.1% Triton X-100; antigen retrieval was by heat mediation in a citrate buffer (pH 6.0). Samples were incubated with primary antibody (1/100) overnight at 4°C. A HRP-conjugated Horse anti-rabbit IgG polyclonal (1/2000) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"