Overview

  • Product nameAnti-BDNF antibodySee all BDNF primary antibodies ...
  • Description
    Sheep polyclonal to BDNF
  • Tested applicationsIHC-FoFr, WB, IHC-P, IHC-Fr, ICC/IF, IHC-FrFlmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis
    Predicted to work with: Zebrafish
  • Immunogen

    A synthetic peptide from human mature BDNF conjugated to an immunogenic carrier protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab75040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/100.
WB Use a concentration of 10 - 50 µg/ml. Predicted molecular weight: 14 , 28 kDa.Can be blocked with Human BDNF peptide (ab102031). This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
IHC-P Use a concentration of 10 - 50 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use a concentration of 10 - 50 µg/ml.
ICC/IF 1/100.
IHC-FrFl 1/100.

Target

  • FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
  • Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
  • Involvement in diseaseCongenital central hypoventilation syndrome
  • Sequence similaritiesBelongs to the NGF-beta family.
  • Post-translational
    modifications
    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abrineurin antibody
    • BDNF antibody
    • BDNF antibody
    • BDNF_HUMAN antibody
    • Brain Derived Neurotrophic Factor antibody
    • Brain-derived neurotrophic factor antibody
    • MGC34632 antibody
    • Neurotrophin antibody
    see all

Anti-BDNF antibody images

  • ab75040 staining cultured rat DRG neurons by ICC/IF. The cultured neurons were fixed with formaldehyde and blocked with 10% donkey serum for 1 hour  at 24°C. The cultured neurons were then stained with ab75040 at 1/100 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 24h at 4°C. An Alexa Fluro 488 donkey anti-sheep polyclonal antibody at 1/1000 was used as the secondary antibody. Beta II tubulin  (which stains the axons) can be observed in red (Alexa fluro 568 secondary). BDNF is observable in the DRG neuron's growth cones.

    See Abreview

  • ab75040 staining human brain (striatum)  sections by IHC-FrFl.  The tissue sections were stained with ab75040 at a 1/100 dilution in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 1h at 24°C. A donkey anti-rabbit Alexa488 polyclonal antibody at 1/1000 was used as the secondary antibody. BDNF expression can be observed in neuronal cell bodies and in processes.

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  • IHC-FoFr image of BDNF staining on rat cortex sections using ab75040 (1:100). The image shows sections incubated with ab75040(right) and without ab75040 (left). The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.

    See Abreview

References for Anti-BDNF antibody (ab75040)

ab75040 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Xenopus laevis Tissue sections (Frozen sections from tecum of Xenopus laevis)
Specification Frozen sections from tecum of Xenopus laevis
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. WANHUA SHEN

Verified customer

Submitted Aug 05 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (cortex)
Specification cortex
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Karine Thibault

Verified customer

Submitted Feb 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (cortex)
Loading amount 30 µg
Specification cortex
Gel Running Conditions Reduced Denaturing (gel 10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Karine Thibault

Verified customer

Submitted Feb 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Brain section)
Specification Brain section
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Dec 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (DRG neurons)
Specification DRG neurons
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Dec 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"