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Product Name
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BDNF antibody
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See all BDNF antibodies (17)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to BDNF
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Immunogen
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Highly pure (>98%) recombinant BDNF (human Brain-Derived Neurotrophic Factor).
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Reacts with
(species key)
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Hu
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Tested applications
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA:To detect BDNF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant BDNF.
Western Blot:To detect BDNF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant BDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Cellular localization
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Secreted
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Research areas
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Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cardiovascular >> Atherosclerosis >> Diabetes associated
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease
Neuroscience >> Neurology process >> Growth and Development >> Neurotrophins
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Relevance
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Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non neuronal cells. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients. In addition, functional studies showed that age-associated changes in BDNF-mediated pathways can enhance inflammation and increase myocardial injury after myocardial infarction in the aging heart.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS, pH 7.4, no preservative, sterile filtered
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BDNF antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to BDNF
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BDNF antibody - more information
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