Anti-BDNF antibody (ab9793)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab9793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA Use at an assay dependent dilution. To detect hBDNF by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. The detection limit is at least 0.2 - 0.4 ng/well of recombinant hBDNF.
WB Use a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant hBDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
Functional Studies Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hBDNF (2.0 µg/ml), a concentration of 0.54-0.81 µg/ml of this antibody is required.

Target

  • FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
  • Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
  • Involvement in diseaseCongenital central hypoventilation syndrome
  • Sequence similaritiesBelongs to the NGF-beta family.
  • Post-translational
    modifications
    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P23560 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Abrineurin antibody
    • BDNF antibody
    • BDNF antibody
    • BDNF_HUMAN antibody
    • Brain Derived Neurotrophic Factor antibody
    • Brain-derived neurotrophic factor antibody
    • MGC34632 antibody
    • MGC34632 antibody
    • Neurotrophin antibody
    see all

References for Anti-BDNF antibody (ab9793)

ab9793 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Rat Tissue lysate - whole (Hippocampus)
Loading amount 30 µg
Specification Hippocampus
Gel Running Conditions Reduced Denaturing (12 ready-gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted Jan 12 2010

Thank you for your enquiry. We are very sorry to hear that you are having problem with these two antibodies. According to the datasheets of these products, ab9793 and ab6200 have only been testes on human samples and we do not know if they recognize...

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To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will fo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"