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Highly pure (>98%) recombinant BDNF (human Brain-Derived Neurotrophic Factor).
For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.
Our Abpromise guarantee covers the use of ab9793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution. To detect hBDNF by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. The detection limit is at least 0.2 - 0.4 ng/well of recombinant hBDNF.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant hBDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions|
|Functional Studies||Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hBDNF (2.0 µg/ml), a concentration of 0.54-0.81 µg/ml of this antibody is required.|
ab9793 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"