Overview
- Product nameAnti-BDNF antibodySee all BDNF primary antibodies ...
- DescriptionRabbit polyclonal to BDNF
- Tested applicationsELISA, WB, Functional Studies more details
- Species reactivityReacts with: Human
- Immunogen
Highly pure (>98%) recombinant BDNF (human Brain-Derived Neurotrophic Factor).
Properties
- FormLyophilised:Reconstitute with 200µl of sterile water.
- Storage instructionsStore at +4°C.
- Storage bufferPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- Isotypeunknown
- Light chain typeunknown
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab9793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: Use at an assay dependent dilution. To detect hBDNF by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. The detection limit is at least 0.2 - 0.4 ng/well of recombinant hBDNF. |
| WB | WB: Use a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant hBDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions |
| Functional Studies | FuncS: Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hBDNF (2.0 µg/ml), a concentration of 0.54-0.81 µg/ml of this antibody is required. |
Target
- FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
- Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
- Involvement in diseaseCongenital central hypoventilation syndrome (CCHS) [MIM:209880]: Rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Note=The disease is caused by mutations affecting the gene represented in this entry.
- Sequence similaritiesBelongs to the NGF-beta family.
- Post-translational
modificationsThe propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG). - Cellular localizationSecreted.
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Database links
- Entrez Gene: 627 Human
- Omim: 113505 Human
- SwissProt: P23560 Human
- Unigene: 502182 Human
Target information above from: UniProt accession
P23560
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Abrineurin antibodyBDNF antibodyBDNF antibody
- BDNF_HUMAN antibodyBrain Derived Neurotrophic Factor antibodyBrain-derived neurotrophic factor antibodyMGC34632 antibodyMGC34632 antibodyNeurotrophin antibody
see all
References for Anti-BDNF antibody (ab9793)
ab9793 has not yet been referenced specifically in any publications.
