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Overview

  • Product nameBDNF protein (Active)See all BDNF proteins and peptides ...
  • Protein descriptionHighly pure (>98%) recombinant human BDNF.
  • Expression hostE. coli

    • Properties

  • Purification notesSterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • FormLyophilised:Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Concentration information loading...
  • Additional notesThe ED50 was determined by its ability to stimulate chick E8 neurite outgrowth. The expected ED50 for this effect is 0.1-1.0 ng/ml.
  • Sequence notesThe active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions: HSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKEGCRG IDKRHWNSQC RTTQSYVRAL TMDSKKRIGW RFIRID TSCV CTLTIKRGR
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab9794 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Other Other: Use at an assay dependent concentration. This peptide can be used with studies using ab9793.
    Cellular activation CellAct: Use a concentration of 0.5 - 1 µg/ml. The expected ED50 is 0.5-1.0 µg/ml for its ability to stimulate the proliferation of Rat C6 cells.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent concentration. Ab9794 can be used as a WB positive control in conjunction with ab6201.
  • Application notesThis peptide can be used with studies using ab9793.

    • Protein info

    • Alternative names
        AbrineurinBDNFBDNF
        BDNF_HUMANBrain Derived Neurotrophic FactorBrain-derived neurotrophic factorMGC34632MGC34632Neurotrophin
      see all
  • FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
  • Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
  • Involvement in diseaseCongenital central hypoventilation syndrome (CCHS) [MIM:209880]: Rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similaritiesBelongs to the NGF-beta family.
  • Post-translational
    modifications    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P23560 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    BDNF protein (Active) images

    • Western blot - Anti-BDNF antibody (ab6201)
      Western blot - Anti-BDNF antibody (ab6201)
      Anti-BDNF antibody (ab6201) at 1 µg/ml + BDNF protein (Active) (ab9794) at 0.1 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 4 minutes

    References for BDNF protein (Active) (ab9794)

    This product has been referenced in:
    • Wong J & Garner B Evidence that truncated TrkB isoform, TrkB-Shc can regulate phosphorylated TrkB protein levels. Biochem Biophys Res Commun 420:331-5 (2012). Read more (PubMed: 22425982) »
    • Samartgis JR  et al. Brain-derived neurotrophic factor facilitates memory consolidation and reconsolidation of a weak training stimulus in the day-old chick. Neurosci Lett 516:119-23 (2012). Functional Studies . Read more (PubMed: 22484543) »

    See all 3 Publications for this product

    Publishing research using ab9794 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab9794 Submit a Question for ab9794

    Displaying 1 - 9 of 9 results for Abreviews and Q&A

    10
    Votes: Highest First

    Customer kindly contacted us regarding reconstitution of ab9794 (BDNF) and ab138787 (proBDNF).


    I heard back from both labs that produce the BDNF and pro-BDNF proteins, and both said that the reconstitution instructions should be followed directly.
    BDNF ab9794: reconstitute in water
    pro BDNF ab138787: reconstitute in PBS

    protein 96% pure
    uses protein to infuse into birds (30 g sparrows) and they die due to this (untested application)
    What are the other 4% impurities?
    What else is in the lyophlized powder (buffer composition before hand)?

    Thank you for contacting us.
    The lab let me know the following:
    The protein is lyophilized with no additives, which means that it is HPLC purified. Therefore, the only buffer that would be present at lyophilization would be water and 0.1% TFA (Tr...

    Read More

    Sehr geehrte Damen und Herren,
    anliegen möchte ich mich nochmals an Sie wenden, um Ihnen den beantworteten Fragebogen zum Thema WB beantwortet zurück zuschicken. Sie finden außerdem in Anhang die Abbildung des Western Blots mit dem Re...

    Read More

    Vielen Dank für Ihre Antwort und für diese weiteren Informationen.

    Vorweg: Den Rabattcode können Sie ab sofort nutzen. Da der vorige unseren Unterlagen zufolge gerade abgelaufen ist, habe ich Ihnen nochmal einen neuen ausgestellt:...

    Read More

    Sehr geehrte Damen und Herren,habe ein Anliegen bzgl. eines bei Ihnen bestellten Antikörpers, den wir mit einem speziellen rekombinanten Protein ebenfalls von Ihrer Firma ausgetestet hatten. Wir haben den AK getestet mit einer AK Verdünnung von ...

    Read More

    Vielen Dank für Ihre Anfrage.

    Es tut mir leid zu hören, dass Sie Probleme mit unserem BDNF-Antikörper und Proteinhaben.

    Bezüglich des Gutscheincodes kann ich Ihnen mitteilen, dass ich die Ausnahme machen konnte, den...

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    WB with ab10505
    sees multiple bands (isoforms, mature form)
    human cell line samples
    will send results
    send discount code for proteins ab9794 and ab113151- to be used in blocking experiment

    Thank you for your phone call. I am sending you the testing discount codes for the 2 proteins for trying them in a blocking experiment - see below.

    Please send me your WB data so that I cantake a look at your results.
    DISCOUNT CODE: xxx for...

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    send protein sequence the protein represents which isoform/variant ?

    Thank you for contacting us. The active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions: HSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKEGCR...

    Read More

    We would like to proceed and purchase the ab9794. When we will do the Western I will send you an image of the gel.  I will wait for your email with the discount code.  

    DISCOUNT CODE:xxxx Expiration date: dd.mm.2012 I am very pleased to hear you would like to use ab9794 and send us an image of your results. This code will give you 1 free protein before the expiration date. To redeem this offer, please submit an Ab...

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    We are looking for a positive control. Can you recommend one?

    Thank you very much for your call. Firstly, I would like to mention that we also have a PC12 cell lysate available that could be used as positive control for ab108383, as stated on the datasheet. I just point this out as a lysate may be of use as a con...

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    Is it possible to reconstitute this protein to a concentration of 1 mg/ml? Customer needs to know this for her particular application.

    Thank you for your enquiry and patience. Our source for this protein has told me that yes, the protein can be reconstituted to a concentration of 1 mg/ml with water. If you have any additional questions, please contact us again.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"