BDNF protein (Active) (ab9794)
Our Abpromise guarantee covers the use of ab9794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Other||Other: Use at an assay dependent concentration. This peptide can be used with studies using ab9793.|
|Cellular activation||CellAct: Use a concentration of 0.5 - 1 µg/ml. The expected ED50 is 0.5-1.0 µg/ml for its ability to stimulate the proliferation of Rat C6 cells.|
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
|WB||WB: Use at an assay dependent concentration. Ab9794 can be used as a WB positive control in conjunction with ab6201.|
- BDNF_HUMANBrain Derived Neurotrophic FactorBrain-derived neurotrophic factorMGC34632MGC34632Neurotrophin
modificationsThe propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG).
BDNF protein (Active) images
Anti-BDNF antibody (ab6201) at 1 µg/ml +
BDNF protein (Active) (ab9794) at 0.1 µg
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutes
References for BDNF protein (Active) (ab9794)
This product has been referenced in:
- Wong J & Garner B Evidence that truncated TrkB isoform, TrkB-Shc can regulate phosphorylated TrkB protein levels. Biochem Biophys Res Commun 420:331-5 (2012). Read more (PubMed: 22425982) »
- Samartgis JR et al. Brain-derived neurotrophic factor facilitates memory consolidation and reconsolidation of a weak training stimulus in the day-old chick. Neurosci Lett 516:119-23 (2012). Functional Studies . Read more (PubMed: 22484543) »