BDNF protein (ab9794)

Product Name 

BDNF protein

Product type 

Proteins and Peptides

Peptide 

Highly pure (>98%) recombinant human BDNF.

Tested applications 
(see key)

CellAct, SDS-PAGE

Application notes 
(see key)

Recommended dilutions
CellAct: The expected ED₅₀ is 0.5-1.0 µg/ml for its ability to stimulate the proliferation of Rat C6 cells.
SDS-PAGE: Use at an assay dependent dilution.
This peptide can be used with studies using ab9793.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Stem Cells >> Stem Cell Cytokines >> Recombinant Human
Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cardiovascular >> Atherosclerosis >> Diabetes associated
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease
Neuroscience >> Neurology process >> Growth and Development >> Neurotrophins

Relevance 

Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non neuronal cells. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients. In addition, functional studies showed that age-associated changes in BDNF-mediated pathways can enhance inflammation and increase myocardial injury after myocardial infarction in the aging heart.

Storage buffer 

n/a

Purification notes 

Sterile filtered
Greater than 96% pure by SDS-PAGE and HPLC analyses.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).

Form 

Lyophilised

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

The ED₅₀ was determined by its ability to stimulate chick E8 neurite outgrowth. The expected ED50 for this effect is 0.1-1.0 ng/ml.

Expression system 

E. coli

Sequence 

The active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions:

HSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKEGCRG IDKRHWNSQC RTTQSYVRAL TMDSKKRIGW RFIRIDTSCV CTLTIKRGR

References for BDNF protein (ab9794)

  This product has been used in:

Trang T et al. P2X4-receptor-mediated synthesis and release of brain-derived neurotrophic factor in microglia is dependent on calcium and p38-mitogen-activated protein kinase activation. J Neurosci 29:3518-28 (2009). PubMed: 19295157

If you publish research using ab9794 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding BDNF protein

Customer FAQs regarding BDNF protein

Protocols for BDNF protein

Price and availability of products related to BDNF protein

BDNF protein - related products: