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Products:Neuroscience >> Neurology process >> Growth and Development >> Neurotrophins
Overview
Product name
BDNF protein
See all BDNF products (3) ...
Protein description
Highly pure (>98%) recombinant human BDNF.
Expression host
E. coli
Properties
Purification notes
Sterile filtered Greater than 96% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Form
Lyophilised:
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration
Concentration information loading...
Additional notes
The ED50 was determined by its ability to stimulate chick E8 neurite outgrowth. The expected ED50 for this effect is 0.1-1.0 ng/ml.
Sequence notes
The active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobi c interactio ns: HSD PARRGEL SVCDSISEW V TAADKKTA VD MSGGTVTVL E KVPVSKGQ LK QYFYETK CNP MGYTKEGCRG IDKRHWNSQ C RTTQSYVR AL TMDSKKR IGW RFIRID TSCV CTLTI KRGR
Research areas
Stem Cells >> Stem Cell Cytokines >> Recombinant Human
Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cardiovascular >> Atherosclerosis >> Diabetes associated
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease
Neuroscience >> Neurology process >> Growth and Development >> Neurotrophins
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Western blot - Anti-BDNF antibody (ab6201)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Other: Use at an assay dependent concentration. (This peptide can be used with studies using ab9793.)
CellAct: Use a concentration of 0.5 - 1 µg/ml.(The expected ED50 is 0.5-1.0 µg/ml for its ability to stimulate the proliferation of Rat C6 cells.)
SDS-PAGE: Use at an assay dependent dilution.
WB: Use at an assay dependent concentration. (Ab9794 can be used as a WB positive control in conjunction with ab6201. )
Application notes
This peptide can be used with studies using ab9793.
Protein info
Function
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Tissue specificity
Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
Involvement in disease
Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
Sequence similarities
Belongs to the NGF-beta family.
Post-translational
modifications
The propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG).
Cellular localization
Secreted.
Target information above from: UniProt accessionP23560
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
BDNF protein images:
Western blot - Anti-BDNF antibody (ab6201)
Anti-BDNF antibody (ab6201) at 1 µg/ml + BDNF protein (ab9794) at 0.1 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutes
References for BDNF protein (ab9794)
This product has been referenced in:
- Trang Tet al. P2X4-receptor-mediated synthesis and release of brain-derived neurotrophic factor in microglia is dependent on calcium and p38-mitogen-activated protein kinase activation. J Neurosci 29:3518-28 (2009).Read more (PubMed: 19295157) »
See 1 publication for this product
Publishing research using ab9794? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"