Overview

  • Product name
  • Description
    Rabbit polyclonal to BEGAIN
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Dog, Turkey, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Medaka fish
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 543-593 of Human BEGAIN (NP_065887.1).

  • Positive control
    • HeLa whole cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab99945 was affinity purified using an epitope specific to BEGAIN immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 65 kDa.
IP Use at 5-15 µg/mg of lysate.

Target

Anti-BEGAIN antibody images

  • All lanes : Anti-BEGAIN antibody (ab99945) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 65 kDa


    Exposure time : 30 seconds
  • ab99945 at 1 µg/ml detecting BEGAIN in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilized ECL with a 30 second exposure.
    For immunoprecipitation, ab99945 was used at at 10 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1: IP using ab99945
    Lane 2: IP using control IgG.

References for Anti-BEGAIN antibody (ab99945)

ab99945 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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