Overview
- Product nameAnti-BEGAIN antibodySee all BEGAIN primary antibodies ...
- DescriptionRabbit polyclonal to BEGAIN
- Tested applicationsWB, IP more details
- Species reactivityReacts with: Human
- Immunogen
Synthetic peptide, corresponding to a region within amino acids 543-593 of Human BEGAIN (NP_065887.1).
- Positive controlHeLa whole cell lysates
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesab99945 was affinity purified using an epitope specific to BEGAIN immobilized on solid support.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab99945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/2000 - 1/10000. Predicted molecular weight: 65 kDa. |
| IP | IP: Use at 5-15 µg/mg of lysate. |
Target
- FunctionMay sustain the structure of the postsynaptic density (PSD).
- Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Cellular localizationCytoplasm. Membrane.
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Database links
- Entrez Gene: 79146 Human
- Entrez Gene: 57596 Human
- Entrez Gene: 57596 Human
- SwissProt: Q9BUH8 Human
- SwissProt: Q9BUH8 Human
- Unigene: 211751 Human
Target information above from: UniProt accession
Q9BUH8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Begain antibodyBEGIN antibodyBEGIN_HUMAN antibody
- Brain enriched guanylate kinase associated protein antibodyBrain-enriched guanylate kinase-associated homolog (rat) antibodyBrain-enriched guanylate kinase-associated protein antibodyKIAA1446 antibodySAP90/PSD95 Associated Proteins antibodySAPAP antibody
see all
Anti-BEGAIN antibody images
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All lanes : Anti-BEGAIN antibody (ab99945) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
developed using the ECL technique
Predicted band size : 65 kDa
Exposure time : 30 seconds -
ab99945 at 1 µg/ml detecting BEGAIN in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilized ECL with a 30 second exposure.
For immunoprecipitation, ab99945 was used at at 10 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
Lane 1: IP using ab99945
Lane 2: IP using control IgG.
References for Anti-BEGAIN antibody (ab99945)
ab99945 has not yet been referenced specifically in any publications.

