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Anti-BEST4 antibody (ab66632)

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Overview

Product name

Anti-BEST4 antibody

Description

Rabbit polyclonal to BEST4

Tested applications

WBmore details

Cross reactivity

Reacts with

Dog, Human

Predicted to work with

Mouse, Rat, Chicken, Cow

Immunogen

Synthetic peptide: TVSYTLKVAE ARFGGFSGLL LRWRGSIYKL LYKEFLLFGA LYAVLSITYR , corresponding to amino acids 2-51 of Human BEST4

TVSYTLKVAE ARFGGFSGLL LRWRGSIYKL LYKEFLLFGA LYAVLSITYR

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HepG2 cell lysate.

Properties

Form

Lyophilised:
Add 100 µl of distilled water.

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Metabolism >> Plasma Membrane >> Channels

  • Western blot - BEST4 antibody (ab66632)Western blot - BEST4 antibody (ab66632) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab66632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
ELISA titre using peptide based assay: 1/62500.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

BEST4 (Bestrophin 4) is a member of the bestrophin gene family of anion channels. Bestrophins are transmembrane spanning proteins that share a homology region with a high content of aromatic residues including an invariant arginine (R), phenylalanine (F), and proline (P) motif. BEST4 may form calcium-sensitive chloride channels and may conduct other physiologically significant anions such as bicarbonate.

Cellular localization

Cell membrane; Multi-pass membrane protein.

Alternative names

  • BEST 4 antibody
  • Bestrophin 4 antibody
  • Bestrophin4 antibody
  • MGC126872 antibody
  • Vitelliform macular dystrophy 2 like 2 antibody
  • Vitelliform macular dystrophy 2 like protein 2 antibody
  • VMD2L2 antibody
see all

Database links

Anti-BEST4 antibody images:

  Western blot - BEST4 antibody (ab66632)

Western blot - BEST4 antibody (ab66632)

Anti-BEST4 antibody (ab66632) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)


Antibody diluted in 5% skim milk / PBS buffer. Gel concentration 10%.

References for Anti-BEST4 antibody (ab66632)

ab66632 has not yet been referenced specifically in any publications.

Publishing research using ab66632? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"