Anti-MIST-1 antibody (ab187978)
Key features and details
- Rabbit polyclonal to MIST-1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MIST-1 antibody
See all MIST-1 primary antibodies -
Description
Rabbit polyclonal to MIST-1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human MIST-1 aa 100 to the C-terminus.
Database link: Q7RTS1 -
Positive control
- IHC-P: Human pancreas, stomach, tonsil and skeletal muscle tissue; ICC/IF: HepG2 whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
Notes |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Function
Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3'. -
Tissue specificity
Expressed in brain, liver, spleen and skeletal muscle. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Domain
Lacks a classic transcription activation domain and instead possesses an N-terminal region capable of inhibiting heterologous activators. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 168620 Human
- Omim: 608606 Human
- SwissProt: Q7RTS1 Human
- Unigene: 674510 Human
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Alternative names
- Basic helix loop helix domain containing class B 8 antibody
- Basic helix loop helix family member a15 antibody
- BHA15_HUMAN antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling MIST-1 in the nucleus of exocrine glandular cells with ab187978 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immuncytochemical analysis of HepG2 (Human liver hepatocellular carcinoma cell line) whole cells labeling MIST-1 in the nucleoplasm and the Golgi apparatus with ab187978 at 2 µg/ml.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MIST-1 in the nucleus of non-germinal center cells with ab187978 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling MIST-1 in the nucleus of glandular cells with ab187978 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling MIST-1 in the striated muscle fiber (no positivity) with ab187978 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab187978 has been referenced in 7 publications.
- Yoon YJ et al. Salivary gland organoid culture maintains distinct glandular properties of murine and human major salivary glands. Nat Commun 13:3291 (2022). PubMed: 35672412
- Shaalan AK et al. Inhibition of Aurora Kinase B activity disrupts development and differentiation of salivary glands. Cell Death Discov 7:16 (2021). PubMed: 33462217
- Shimizu Y et al. Paneth cell a-defensin misfolding correlates with dysbiosis and ileitis in Crohn's disease model mice. Life Sci Alliance 3:N/A (2020). PubMed: 32345659
- Ingalls MH et al. Intrinsic mitotic activity supports the human salivary gland acinar cell population. FEBS Lett 594:376-382 (2020). PubMed: 31538335
- Shaalan A & Proctor G Salivary glands require Aurora Kinase B for regeneration after transient innate immune-mediated injury. Sci Rep 9:11339 (2019). PubMed: 31383943
- Weng PL et al. Limited Regeneration of Adult Salivary Glands after Severe Injury Involves Cellular Plasticity. Cell Rep 24:1464-1470.e3 (2018). PubMed: 30089258
- Varghese JJ et al. Localized Delivery of Amifostine Enhances Salivary Gland Radioprotection. J Dent Res 97:1252-1259 (2018). PubMed: 29634396