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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-BIN1 antibody [2F11]
See all BIN1 products (9) ...
Description
Mouse monoclonal [2F11] to BIN1
Tested applications
IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
GST chimeric protein that encoded human Bin1 BAR domain.
Positive control
Human Prostate Cancer tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
2F11
Isotype
IgG1
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
IHC-Fr
Application notes
IHC-P: 1/40 for 60 mins at room temperature, using a Biotin based system. For optimal staining, the sections should be pretreated with an antigen unmasking solution.
IHC-Fr: 1/40 for 60 mins at room temperature, using a Biotin based system.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.
Tissue specificity
Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
Involvement in disease
Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
Sequence similarities
Contains 1 BAR domain.
Contains 1 SH3 domain.
Post-translational
modifications
Phosphorylated by protein kinase C.
Cellular localization
Cytoplasm and Nucleus.
Target information above from: UniProt accessionO00499
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Amphiphysin like protein antibody
- AMPH 2 antibody
- AMPH2 antibody
see all
Database links
- Entrez Gene: 274 Human
- Omim: 601248 Human
- SwissProt: O00499 Human
- Unigene: 193163 Human
References for Anti-BIN1 antibody [2F11] (ab84974)
ab84974 has not yet been referenced specifically in any publications.
Publishing research using ab84974? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"