Anti-BIN1 antibody (ab106937)

Overview

• Product nameAnti-BIN1 antibodySee all BIN1 primary antibodies ...
• Description
  Goat polyclonal to BIN1
• Specificityab106937 is expected to recognize isoform 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 (NP_647593.1, NP_647594.1, NP_647595.1, NP_647596.1, NP_647597.1, NP_647598.1, NP_647599.1, NP_004296.1, NP_647600.1, NP_647601.1).
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Cow, Pig
• Immunogen

  Synthetic peptide: C-SDNAPAKGNKSPS, corresponding to internal sequence amino acids 286-298 of Human BIN1 (NP_647593.1)

  Run BLAST with Run BLAST with
• Positive controlHuman skeletal muscle lysates

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cell cycle inhibitors
  • Other

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • Other

  • Microbiology
  • Interspecies Interaction
  • Host Virus Interaction

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Cancer susceptibility
  • Tumor Suppressors

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Cycle Inhibitors
  • Other

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • Other

Applications

Target

• FunctionMay be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.
• Tissue specificityUbiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
• Involvement in diseaseDefects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
• Sequence similaritiesContains 1 BAR domain.
  Contains 1 SH3 domain.
• Post-translational
  modificationsPhosphorylated by protein kinase C.
• Cellular localizationCytoplasm and Nucleus.

Target information above from: UniProt accession O00499 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 614576 Cow
  • Entrez Gene: 274 Human
  • Entrez Gene: 30948 Mouse
  • Entrez Gene: 100037305 Pig
  • Omim: 601248 Human
  • SwissProt: O00499 Human
  • SwissProt: O08539 Mouse
  • Unigene: 193163 Human

  • Unigene: 4383 Mouse

  see all

• Alternative names
  AMPH 2 antibodyAMPH2 antibodyAmphiphysin 2 antibody

  Amphiphysin II antibodyAmphiphysin like protein antibodyamphiphysin-like antibodyAmphiphysin-like protein antibodyAMPHL antibodyBin1 antibodyBIN1_HUMAN antibodyBox Dependant MYC Interacting Protein 1 antibodyBox-dependent myc-interacting protein 1 antibodyBridging Integrator 1 antibodyDKFZp547F068 antibodyMGC10367 antibodyMGC105358 antibodyMyc box dependent interacting protein 1 antibodyMyc box-dependent-interacting protein 1 antibodySH3P9 antibody

  see all

Anti-BIN1 antibody images

• 

  Western blot - BIN1 antibody (ab106937)
  Anti-BIN1 antibody (ab106937) at 0.1 µg/ml + Human skeletal muscle lysate (in RIPA buffer) at 35 µg
  developed using the ECL technique
  
  Predicted band size : 65 kDa
  Observed band size : 60 + 70 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 1 hour