Anti-BIN1 antibody (ab153912)
Key features and details
- Rabbit polyclonal to BIN1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-BIN1 antibody
See all BIN1 primary antibodies -
Description
Rabbit polyclonal to BIN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human BIN1 aa 1-300.
Database link: O00499-1 -
Positive control
- A431 and Mouse kidney whole cell lysates; Human U87 xenograft tissue; A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 65 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 65 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. -
Tissue specificity
Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle. -
Involvement in disease
Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers. -
Sequence similarities
Contains 1 BAR domain.
Contains 1 SH3 domain. -
Post-translational
modificationsPhosphorylated by protein kinase C. -
Cellular localization
Cytoplasm and Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 274 Human
- Entrez Gene: 30948 Mouse
- Omim: 601248 Human
- SwissProt: O00499 Human
- SwissProt: O08539 Mouse
- Unigene: 193163 Human
- Unigene: 4383 Mouse
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Alternative names
- AMPH 2 antibody
- AMPH2 antibody
- Amphiphysin 2 antibody
see all
Images
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Anti-BIN1 antibody (ab153912) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 65 kDa
10% SDS PAGE -
Anti-BIN1 antibody (ab153912) at 1/500 dilution + Mouse kidney whole cell lysate at 50 µg
Predicted band size: 65 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue labeling BIN1 with ab153912 at 1/500 dilution.
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Immunofluorescent analysis of A431 cells (fixed in iced cold MeOH; 5 mins) labeling BIN1 with ab153912 at 1/500 dilution (green). Right image shows cells co-stained with Hoechst 33342 (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab153912 has been referenced in 2 publications.
- Crotti A et al. BIN1 favors the spreading of Tau via extracellular vesicles. Sci Rep 9:9477 (2019). PubMed: 31263146
- McAvoy KM et al. Cell-autonomous and non-cell autonomous effects of neuronal BIN1 loss in vivo. PLoS One 14:e0220125 (2019). PubMed: 31408457