Anti-BIN1 antibody (ab27796)
Key features and details
- Rabbit polyclonal to BIN1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BIN1 antibody
See all BIN1 primary antibodies -
Description
Rabbit polyclonal to BIN1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Mouse BIN1. Corresponding to region surrounding amino acid 578
Database link: O08539-1 -
Positive control
- Jurkat cells
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 3672 Bin1 Antibody. 3672-100 is the same size as the 100 µg size of ab27796.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Notes |
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Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. -
Tissue specificity
Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle. -
Involvement in disease
Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers. -
Sequence similarities
Contains 1 BAR domain.
Contains 1 SH3 domain. -
Post-translational
modificationsPhosphorylated by protein kinase C. -
Cellular localization
Cytoplasm and Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 274 Human
- Omim: 601248 Human
- SwissProt: O00499 Human
- Unigene: 193163 Human
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Alternative names
- AMPH 2 antibody
- AMPH2 antibody
- Amphiphysin 2 antibody
see all
Images
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All lanes : Anti-BIN1 antibody (ab27796) at 2 µg/ml
Lane 1 :Jurkat whole cell lysate (ab7899)
Lane 2 : Jurkat cells treated with camptothecin for 6 hours
Predicted band size: 65 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa (possible cleavage fragment)
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab27796 has been referenced in 2 publications.
- Lambert E et al. The Alzheimer susceptibility gene BIN1 induces isoform-dependent neurotoxicity through early endosome defects. Acta Neuropathol Commun 10:4 (2022). PubMed: 34998435
- Chapuis J et al. Increased expression of BIN1 mediates Alzheimer genetic risk by modulating tau pathology. Mol Psychiatry N/A:N/A (2013). WB ; Human . PubMed: 23399914