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used rat heart lysate as sample as in QC image; used up to 1ug/ml primary, 4x more than in QC image; no bands detected, secondary OK; detected other targets using same lysate as wb sample; |
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ANSWER: |
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab27796 with the order number 1080280. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-BIN1 antibody (ab27796) at 2 µg/ml
Lane 1 :
Lane 2 : Jurkat cells treated with camptothecin for 6 hours
Predicted band size : 65 kDa
Observed band size : 64 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa (possible cleavage fragment).
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