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Overview

  • Product nameBIN1 protein (Tagged-His Tag)See all BIN1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 96-439 of Human BIN1 with N-terminal His tag, Predicted MWt 39 kDa (UniProt O00499).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 118 µl aqua dest
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium hydrogen phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesGRDEANKIAENNDLLWMDYHQKLVDQALLTMDTYLGQFPD IKSRIAKRGRK LVDYDSARHHYESLQTAKKKDEAKIAK AEEELIKAQKVFEEMNVDLQEEL PSLWNSRVGFYVNTF QSIAGLEENFHKEMSKLNQNLNDVLVGLEKQHGSN TFT VKAQPSDNAPAKGNKSPSPPDGSPAATPEIRVNHEPEPAG GATPGAT LPKSPSQPAEASEVAGGTQPAAGAQEPGETA ASEAASSSLPAVVVETFPA TVNGTVEGGSGAGRLDLPP GFMFKVQAQHDYTATDTDELQLKAGDVVLVI PFQNPEE QDEGWLMGVKESDWNQHKELEKCRGVFPENFTERVP
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91662 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        AMPH 2AMPH2Amphiphysin 2
        Amphiphysin IIAmphiphysin like proteinamphiphysin-likeAmphiphysin-like proteinAMPHLBin1BIN1_HUMANBox Dependant MYC Interacting Protein 1Box-dependent myc-interacting protein 1Bridging Integrator 1DKFZp547F068MGC10367MGC105358Myc box dependent interacting protein 1Myc box-dependent-interacting protein 1SH3P9
      see all
  • FunctionMay be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.
  • Tissue specificityUbiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
  • Involvement in diseaseDefects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
  • Sequence similaritiesContains 1 BAR domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications    Phosphorylated by protein kinase C.
  • Cellular localizationCytoplasm and Nucleus.

    • Target information above from: UniProt accession O00499 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for BIN1 protein (Tagged-His Tag) (ab91662)

    ab91662 has not yet been referenced specifically in any publications.

    Publishing research using ab91662 ? Please let us know so that we can cite the reference in this datasheet

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