Anti-BLBP antibody - Neuronal Marker (ab32423)
Overview
- Product nameAnti-BLBP antibody - Neuronal MarkerSee all BLBP primary antibodies ...
- DescriptionRabbit polyclonal to BLBP - Neuronal Marker
- Tested applicationsIHC-FoFr, IHC-P, ICC/IF, WB more details
- Species reactivityReacts with: Mouse, Rat, Chicken, Zebrafish, Quail
Predicted to work with: Human, Pig - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse BLBP.
(Peptide available as ab32422.)
- Positive controlRat spinal cord from embryonic day 16; young zebrafish spinal cord This antibody gave a positive signal in the following mouse lysate: Brain (Mouse) Tissue Lysate - normal tissue, 0 days old
Properties
- FormLiquid
- Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab32423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-FoFr | |
| IHC-P | |
| ICC/IF | |
| WB |
IHC-P: 1/200 - 1/1000.
IHC-FoFr: 1/2000 (PMID 19094094).
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionB-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers.
- Tissue specificityExpressed in brain and other neural tissues.
- Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
- DomainForms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
- Cellular localizationCytoplasm.
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Database links
- Entrez Gene: 396246 Chicken
- Entrez Gene: 2173 Human
- Entrez Gene: 12140 Mouse
- Entrez Gene: 80841 Rat
- Omim: 602965 Human
- SwissProt: Q05423 Chicken
- SwissProt: O15540 Human
- SwissProt: P51880 Mouse
- SwissProt: P55051 Rat
- Unigene: 26770 Human
- Unigene: 3644 Mouse
- Unigene: 10014 Rat
see all
Target information above from: UniProt accession
O15540
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- B FABP antibodyB-FABP antibodyBFABP antibody
- BLBP antibodyBrain lipid binding protein antibodyBrain lipid-binding protein antibodyBrain-type fatty acid-binding protein antibodyDKFZp547J2313 antibodyFABP 7 antibodyFABP7 antibodyFABP7_HUMAN antibodyFABPB antibodyFatty Acid Binding Protein 7 antibodyFatty acid binding protein 7 brain antibodyFatty acid binding protein brain antibodyFatty acid-binding protein 7 antibodyFatty acid-binding protein, brain antibodyMammary derived growth inhibitor related antibodyMammary-derived growth inhibitor related antibodyMRG antibodyOTTHUMP00000017119 antibody
see all
Anti-BLBP antibody - Neuronal Marker images
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Immunohistochemistry (Paraffin-embedded sections) - BLBP antibody (ab32423)Carl hobbs, KCL, UKBLBP detected on rat embryo day 16 spinal cord T/S on 5 micron thick formalin-fixed paraffin wax embedded sections using Rabbit polyclonal to BLBP (ab32423; incubated at 1/200 for 16h at RT). 1% BSA (10 mins at RT) used as blocking agent. Secondary Antibody was biotin labeled anti-rabbit IgG (1/300). Immunoreactivity was visualised by standard streptAvidin-biotin-complex using peroxidase as a reporter and DAB as chromogen following citric acid (pH 6) heat mediated antigen retrieval. Expected specific staining observed with ab32423 with low signal to noise ratio. -
Western blot - BLBP antibody (ab32423)Anti-BLBP antibody - Neuronal Marker (ab32423) at 1/250 dilution +Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 15 kDa
Observed band size : 15 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BLBP antibody - Neuronal Marker (ab32423)Carl Hobbs, CARD, KCL, UKBLBP detected on mouse embryo day 16 spinal cord T/S on 5 micron thick formalin-fixed paraffin wax embedded sections using Rabbit polyclonal to BLBP (ab32423; incubated at 1/300 for 16h at RT). 1% BSA (10 mins at RT) used as blocking agent. Secondary Antibody was biotin labeled anti-rabbit IgG (1/300). Immunoreactivity was visualised by standard streptAvidin-biotin-complex using peroxidase as a reporter and DAB as chromogen following citric acid (pH 6) heat mediated antigen retrieval. Expected specific staining observed with ab32423. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BLBP antibody - Neuronal Marker (ab32423)Carl Hobbs, CARD, KCL, London, UKImmunohistochemistical staining (Formaldehyde/PFA-fixed paraffin-embedded sections) for BLBP antibody - Neuronal Marker (ab32423)on Zebrafish Tissue sections (young adult whole body T/S). Antigen retrieval step: Heat mediated Blocking step: 1% BSA for 10 mins at RT. Primary Antibody ab32423 incubated at 1/500 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BLBP antibody - Neuronal Marker (ab32423)Carl Hobbs, CARD, KCL, London, UKImmunohistochemistical detection (on Formaldehyde /PFA-fixed paraffin-embedded sections) of BLBP protein using BLBP antibody - Neuronal Marker (ab32423) on Quail Tissue sections (developing spinal cord). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody ab32423 incubated at 1/500 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300). -
Immunocytochemistry/ Immunofluorescence - BLBP antibody - Neuronal Marker (ab32423)ICC/IF image of ab32423 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab32423, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-BLBP antibody - Neuronal Marker (ab32423)
This product has been referenced in:
- De Cegli R et al. Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation. Nucleic Acids Res 41:711-726 (2013). ICC/IF, IHC ; Mouse . Read more (PubMed: 23180766) »
- Tian C et al. Foxg1 has an essential role in postnatal development of the dentate gyrus. J Neurosci 32:2931-49 (2012). IHC-FoFr ; Mouse . Read more (PubMed: 22378868) »
