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Anti-BLMH antibody (ab77111) at 1/500 dilution + recombinant fragment of human BLMH (the immunogen) at 0.2 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
Predicted band size : 53 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Molecular weight of the tag alone is 26 kDa.
Anti-BLMH antibody (ab77111) at 1/500 dilution + K562 cell lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa
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