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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-BLMH antibody
See all BLMH products (3) ...
Description
Mouse monoclonal to BLMH
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Recombinant fragment: NMNKAERLTF GESLMTHAMT FTAVSEKDDQ DGAFTKWRVE NSWGEDHGHK GYLCMTDEWF SEYVYEVVVD RKHVPEEVLA VLEQEPIILP AWDPMGALA, corresponding to amino acids 356-454 of human BLMH (NP_000377) with a 26 kDa tag
NMNKAERLTF GESLMTHAMT FTAVSEKDDQ DGAFTKWRVE NSWGEDHGHK GYLCMTDEWF SEYVYEVVVD RKHVPEEVLA VLEQEPIILP AWDPMGALA
Positive control
K562 cell lysate; recombinant tagged fragment of human BLMH (the immunogen).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. (Detection limit for recombinant tagged BLMH is approximately 0.3ng/ml when used as a capture antibody.)
WB: 1/500 - 1/1000.Predicted molecular weight: 53 kDa.
Target
Relevance
BLMH is a member of the peptidase C1 family and exists as a homohexamer. While its normal physiological function is not known, it protects normal and malignant cells from the glycopeptide antitumor drug BLM. It inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine and also shows general aminopeptidase activity. The specificity varies but amino acid arylamides of Met, Leu and Ala are preferred.
Cellular localization
Cytoplasmic
Alternative names
- BH antibody
- bleomycin hydrolase antibody
- BLM hydrolase antibody
see all
Database links
- Entrez Gene: 642 Human
- Entrez Gene: 104184 Mouse
- Entrez Gene: 287552 Rat
- Omim: 602403 Human
- SwissProt: Q13867 Human
- SwissProt: Q8R016 Mouse
- SwissProt: P70645 Rat
- Unigene: 371914 Human
see all
Anti-BLMH antibody images:
Western blot - BLMH antibody (ab77111)
Anti-BLMH antibody (ab77111) at 1/500 dilution + recombinant fragment of human BLMH (the immunogen) at 0.2 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
Predicted band size : 53 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Molecular weight of the tag alone is 26 kDa.
Western blot - BLMH antibody (ab77111)
Anti-BLMH antibody (ab77111) at 1/500 dilution + K562 cell lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa
References for Anti-BLMH antibody (ab77111)
ab77111 has not yet been referenced specifically in any publications.
Publishing research using ab77111? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"