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Overview

  • Product nameBLMH protein (Tagged-His Tag)See all BLMH proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 309-455 of Human BLMH with N terminal His tag; 147 amino acids, 20kDa (17kDa predicted from sequence). UniProt ID = Q13867.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 98 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesKKMVAASIKDGEAVWFGCDVGKHFNSKLGLSDMNLYDHEL VFGVSLKNMNKAERLTF GESLMTHAMTFTAVSEKDDQD GAFTKWRVENSWGEDHGHKGYLCMTDEWFSEYVYEV VV DRKHVPEEVLAVLEQEPIILPAWDPMGALAE
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91900 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        BHbleomycin hydrolaseBLM hydrolase
        BLMHBLMH_HUMANBMHEC 3.4.22.40
      see all
  • FunctionThe normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.
  • Sequence similaritiesBelongs to the peptidase C1 family.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession Q13867 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    BLMH protein (Tagged-His Tag) images

    • SDS-PAGE - BLMH protein (Tagged-His Tag) (ab91900)
      SDS-PAGE - BLMH protein (Tagged-His Tag) (ab91900)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for BLMH protein (Tagged-His Tag) (ab91900)

    ab91900 has not yet been referenced specifically in any publications.

    Publishing research using ab91900 ? Please let us know so that we can cite the reference in this datasheet

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