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Product name
Anti-BLNK antibody
See all BLNK products (7) ...
Description
Goat polyclonal to BLNK
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide: KDSTRLKYAVKVS, corresponding to C terminal amino acids 444-456 of Human BLNK.
C-KDSTRLKYAVKVS
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Adapters >> Transmembrane
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
WB
Application notes
WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 51 kDa. Approx 70kDa band seen in Daudi lysate.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays an important role in BCR-induced B-cell apoptosis.
Tissue specificity
Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
Involvement in disease
Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) [MIM:613502]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
Sequence similarities
Contains 1 SH2 domain.
Post-translational
modifications
Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.
Cellular localization
Cytoplasm. Cell membrane. BCR activation results in the translocation to membrane fraction.
Target information above from: UniProt accessionQ8WV28
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B cell adapter containing SH2 domain protein antibody
- B cell adapter containing Src homology 2 domain protein antibody
- B cell linker antibody
see all
Database links
- Entrez Gene: 29760 Human
- Entrez Gene: 17060 Mouse
- NCBI: 7019535 Human
- Omim: 604515 Human
- SwissProt: Q8WV28 Human
- SwissProt: Q9QUN3 Mouse
- Unigene: 665244 Human
- Unigene: 9749 Mouse
Anti-BLNK antibody images:
Western blot - BLNK antibody (ab2230)
Anti-BLNK antibody (ab2230) at 4 µg/ml + Cell lysate prepared from human Daudi cells at 35 µg
developed using the ECL technique
Predicted band size : 51 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Primary incubated for 1 hour.
Western blot - BLNK antibody (ab2230)
All lanes : Anti-BLNK antibody (ab2230) at 1 µg/ml
Lane 1 : Cell lysate prepared from HEK293 mock transfected cells
Lane 2 : Cell lysate prepared from HEK293 overexpressing BLNK
Predicted band size : 51 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
References for Anti-BLNK antibody (ab2230)
ab2230 has not yet been referenced specifically in any publications.
Publishing research using ab2230? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"