Anti-BMP1 antibody - CUB-2 domain (ab38952)

Thanks for getting back to me with this information. We actually want to use this antibody to detect endogenous BMP-1 in HepG2 cells by western blot, will we still be covered under the Abpromise guarantee for this? If so then I will look into ordering the product and we will test it on our cell lysates.
Thanks

ANSWER:

Thank you for getting back to me.

Yes, the antibody would be covered by the Abpromise for the experiment you describe. If you have any problems please do let us know. I would strongly encourage you to also submit an Abreview once you have tried the antibody. This will only take 5 minutes and will enable other customers to see how you got on and if the antibody is suitable for them. As a reward you will receive Abpoints which can go towards subsequent purchases with Abcam, or be exchanged for Amazon vouchers.

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us.

Phone call requesting information on if the antibody had been used to detect endogenously expressed protein for western blotting.

ANSWER:

I am sorry for the delay in getting back to you in regards to anti-BMP1 antibody (ab38952).

I now have some further information to share with you in regards to this antibody. The western blot we have performed with this antibody is from using the secreted protein. I am still trying to define if this was from endogenously expressed samples, or from transiently expressed. However, I can confirm, as I suspected, that the HepG2 cells stained with the antibody has been used to detect the endogenously expressed protein. The following reference has also used the antibody in order to detect the protein in ventricular specimens using western blotting:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2857990/?tool=pubmed

The authors have noted four isoforms of BMP1 being detected between 70-100 kDa but it appears that there is some non-specific background signals observed. This could conceivably be improved by decreasing the amount of sample loaded as well as antibody used, and optimising the blocking conditions used (I would suggest switching to 3% BSA in TBST instead of 5% milk)

I would like to reassure you that if you were to use this antibody in western blotting with human samples and you found it was not able to detect the endogenously expressed protein, you would be eligible for a refund or a replacement antibody under the Abpromise guarantee. More information on this can be found from the following link:

http://www.abcam.com/abpromise

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.