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Read our guarantee »Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
Anti-BMP1 antibody - Carboxyterminal end
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Rabbit polyclonal to BMP1 - Carboxyterminal end
ab65919 is made to the carboxyterminal end of the 823 amino acid splice variant of human BMP1, after the his-rich region, and before the furin cleavage site. The antibody is made to an area specific for this isoform of BMP1, and will not detect longer forms or the tolloid-like forms.
Reacts with
Human
Synthetic peptide based on the carboxyterminal end of the isoform 2 splice variant of the human sequence.
Extracts of HDFn treated with TNF-alpha.
Liquid
Store at -20°C. Stable for 12 months at -20°C
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> BMPs
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
Western blot - BMP1 antibody - Carboxyterminal end (ab65919)
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Our Abpromise guarantee covers the use of ab65919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 180 kDa (predicted molecular weight: 99 kDa).
EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Bone morphogenetic protein 1 (BMP1) was first identified in osteogenic extracts of bone. It is an extracellular zinc endopeptidase, implicated in morphogenetic processes in a broad range of species. BMP1 is a member of the astacin family of metalloproteinases. The astacin family includes BMP1, astacin, meprin A and B, tolloid-like proteins, and choriolysin. BMP1 is involved in extracellular matrix (ECM) formation, suggesting that a functional link may exist between astacin metalloproteinases, growth factors, and cell differentiation and pattern formation during development. The name PCP reflects this enzyme’s involvement in the collagen deposition of growing bone. The enzymes known as the procollagen C and N proteinases (PCP and PNP) are involved in the processing of fibrillar procollagen precursors to mature collagens, which is an essential requirement for fibril formation. PCP cleaves the C-terminus from procollagen, to allow the formation of mature, triplehelical collagen. The N-terminus is cleaved by the procollagen N-proteinase (PNP or ADAM-TS2). Defects in PNP have been linked to the skin disorder dermatosparaxis, and defects in BMP1 are thought to lead to aberrant collagen processing, and connective tissue disorders. Many forms of BMP1 have been reported, with varying truncation at the C-terminus. The long form of BMP1 is most similar to the tolloid-like proteins, which have extra EGF-like and CUB domains.
Secreted
Western blot - BMP1 antibody - Carboxyterminal end (ab65919)

All lanes : Anti-BMP1 antibody - Carboxyterminal end (ab65919) at 1/1000 dilution
Lane 1 : High molecular weight markers
Lane 2 : HDFn treated with TNF-alpha in Complete Medium at 20 µl
Lane 3 : HDFn treated with TNF-alpha in Complete Medium at 25 µl
Lane 4 : HDFn treated with TNF-alpha in Complete Medium at 30 µl
Predicted band size : 99 kDa
Observed band size : 180 kDa (why is the actual band size different from the predicted?)
ab65919 has not yet been referenced specifically in any publications.
Publishing research using ab65919? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-BMP1 antibody - Carboxyterminal end (ab65919) at 1/1000 dilution
Lane 1 : High molecular weight markers
Lane 2 : HDFn treated with TNF-alpha in Complete Medium at 20 µl
Lane 3 : HDFn treated with TNF-alpha in Complete Medium at 25 µl
Lane 4 : HDFn treated with TNF-alpha in Complete Medium at 30 µl
Predicted band size : 99 kDa
Observed band size : 180 kDa (why is the actual band size different from the predicted?)
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