Overview
- Product nameBMP2 Mouse ELISA kitSee all BMP2 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
- Assay typeSandwich
- Sensitivity< 2 pg/ml
- Range62.5 pg/ml - 4000 pg/ml
- Species reactivityReacts with: Mouse
- Product overview
ab119582 BMP2 Mouse ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse BMP2 specific-specific monoclonal antibodies were precoated onto 96-well plates. The mouse specific detection monoclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BMP2 amount of sample captured in plate.
No detectable cross-reactivity with any other cytokine.
- Notes
Store at 4°C for frequent use, at -2°C for infrequent use. Avoid multiple freeze-thaw cycles.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 96-well plate precoated with anti-mouse BMP2 antibody (12 strips) 1 unit ABC diluent buffer 1 x 12ml Antibody diluent buffer 1 x 12ml Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-mouse BMP2 antibody 1 x 130µl Lyophilized recombinant mouse BMP2 standard 2 vials Sample diluent buffer 1 x 30ml TMB color developing agent 1 x 10ml TMB stop solution 1 x 10ml -
Research Areas
Target information above from: UniProt accession
P12643
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- BDA2BMP-2BMP-2A
- Bmp2BMP2_HUMANBMP2ABone morphogenetic protein 2Bone morphogenetic protein 2A
see all
- Entrez Gene: 12156 Mouse
- SwissProt: P21274 Mouse
Applications
Our Abpromise guarantee covers the use of ab119582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
BMP2 Mouse ELISA kit images
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Sandwich ELISA - BMP2 Mouse ELISA kit (ab119582)Representative standard curve using ab119582.
Protocols
References for BMP2 Mouse ELISA kit (ab119582)
ab119582 has not yet been referenced specifically in any publications.
