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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BMP2 antibody [65529.111] (ab6285)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BMP2 antibody [65529.111] (ab6285)](/ps/datasheet/images/6/ab6285/BMP2-Primary-antibodies-ab6285-2.jpg)
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Product Name
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BMP2 antibody [65529.111]
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See all BMP2 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [65529.111] to BMP2
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Immunogen
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Recombinant full length protein expressed in E. coli (Human).
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Reacts with
(species key)
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Hu, Ms
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Specificity
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Monoclonal anti-BMP-2 recognizes recombinant human BMP-2 by ELISA and immunoblotting.
The antibody shows approximately 25% cross-reactivity with recombinant human BMP-4.
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Tested applications
(see key)
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ELISA, I-ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at an assay dependent dilution PMID 17959825. Do not perform antigen retrieval.
I-ELISA: Use at a concentration of 0.5 - 1 µg/ml to detect a limit of ~3 ng/well recombinant human BMP-2 at 50 and 100 ng/lane under non-reducing and reducing conditions, respectively.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 17-18 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Cancer >> Growth factors >> TGF
Stem Cells >> Neural Stem Cells >> Extracellular
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Growth Factors/Hormones >> TGF
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Relevance
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BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily of secreted growth factors. It is a disulfide-linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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65529.111
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Isotype
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IgG2b
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Purity
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Protein G purified
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Storage buffer
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Phosphate buffered saline
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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This product is supplied as 100ug/vial. The concentration range may vary from 0.5mg/ml to 1mg/ml.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BMP2 antibody [65529.111] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BMP2 antibody [65529.111] images, references, products related to ab6285 and other tools.
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BMP2 antibody [65529.111] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BMP2 antibody [65529.111] (ab6285)
ab66285 staining BMP2 in Human small intestine tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1.5% serum for 10 minutes. Antigen retrieval was by heat mediation in citrate (pH6.0). Samples were incubated with primary antibody (1/100 in diluent buffer) for 1 hour. An undiluted HRP-conjugated Goat polyclonal to mouse IgG was used as secondary antibody This image is courtesy of an anonymous Abreview
See Abreview
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References for BMP2 antibody [65529.111] (ab6285)
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This product has been used in: (two most recent references)
Segrelles C et al. Constitutively Active Akt Induces Ectodermal Defects and Impaired Bone Morphogenetic Protein Signaling. Mol Biol Cell 19:137-149 (2008). IHC-P; Mouse. PubMed: 17959825
Yew KH et al. Cross-talk between bone morphogenetic protein and transforming growth factor-beta signaling is essential for exendin-4-induced insulin-positive differentiation of AR42J cells. J Biol Chem 280:32209-17 (2005). PubMed: 16020542
If you publish research using ab6285 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to BMP2
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BMP2 antibody [65529.111] - more information
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Customer reviews (feedback) regarding BMP2 antibody [65529.111] |
Customer FAQs regarding BMP2 antibody [65529.111] |
Protocols for BMP2 antibody [65529.111] |
Price and availability of products related to BMP2 antibody [65529.111] |
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BMP2 antibody [65529.111] - related products:
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BMP2 antibody [65529.111] - other tools:
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Contact Abcam with a Technical Enquiry about BMP2 antibody [65529.111] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BMP2 antibody [65529.111] (ab6285)](/index.html?datasheet=6285#BMP2-Primary-antibodies-ab6285-2.jpg)
