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Needs the MSDS for ab17885. |
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ANSWER: |
Thank you for your call today. I have attached the MSDS for ab17885. Please let me know if you have any questions or if there is anything else that we can do for you. |
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Dear Sir/Madam Please kindly recommend secondary ab for rabbit polyclonal to BMP2 (ab17885) Application: WB. Conjugate :AP. Best regards |
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ANSWER: |
Thank you for your enquiry and your interest in our products. I would advise you or your customer to conduct a search using our advanced search facility specifically designed for secondary antibodies: http://www.abcam.com/index.html?pageconfig=productmap&cl=918 You can select isotype, target species, host species, conjugate, application, clonality and find the most suitable and compatible antibodies from our current secondary product range (2,298). I hope this helps and if I can assist further, please do not hesitate to contact me. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ICC/IF image of ab17885 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17885, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-BMP2 antibody - Differentiation marker (ab17885) at 0.1 µg/ml +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
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