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Products:Signal Transduction >> Growth Factors/Hormones >> TGF
Overview
Product name
Anti-BMP2 antibody - Differentiation marker
See all BMP2 products (7) ...
Description
Rabbit polyclonal to BMP2 - Differentiation marker
Tested applications
ICC/IF, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
E.coli derived Human BMP2, mature form. The amino acid sequence: MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR.
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPP GYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNS KIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Growth factors >> TGF
Stem Cells >> Neural Stem Cells >> Extracellular
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Growth Factors/Hormones >> TGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab17885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
ELISA: Use a concentration of 0.5 µg/ml
WB: Use a concentration of 0.1 - 0.2 µg/ml.Predicted molecular weight: 45 kDa.
Target
Function
Induces cartilage and bone formation.
Tissue specificity
Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.
Sequence similarities
Belongs to the TGF-beta family.
Cellular localization
Secreted.
Target information above from: UniProt accessionP12643
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BMP 2 antibody
- BMP 2A antibody
- BMP-2 antibody
see all
Database links
- Entrez Gene: 650 Human
- Omim: 112261 Human
- SwissProt: P12643 Human
- Unigene: 73853 Human
Anti-BMP2 antibody - Differentiation marker images:
Immunocytochemistry/ Immunofluorescence-BMP2 antibody - Differentiation marker(ab17885)
ICC/IF image of ab17885 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17885, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot
Anti-BMP2 antibody - Differentiation marker (ab17885) at 0.1 µg/ml + BMP2 protein (Human) (ab87065) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
References for Anti-BMP2 antibody - Differentiation marker (ab17885)
ab17885 has not yet been referenced specifically in any publications.
Publishing research using ab17885? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"