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Immunohistochemistry (Frozen sections) - BMP2 antibody (ab14933)
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Immunocytochemistry/ Immunofluorescence - BMP2 antibody (ab14933)
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Immunocytochemistry/ Immunofluorescence - BMP2 antibody (ab14933)
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Product Name
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BMP2 antibody
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See all BMP2 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to BMP2
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Immunogen
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Synthetic peptide: PSDEVLSEFELRLLSM conjugated to KLH, corresponding to amino acids 45-60 of BMP2(Peptide available as ab80798.)
BLAST 'PSDEVLSEFELRLLSM' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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Dot, ELISA, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Dot: 1/2000.
ELISA: 1/2000.
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 45 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: neonatal brain lysate
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Cellular localization
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Secreted
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Research areas
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Cancer >> Growth factors >> TGF
Stem Cells >> Neural Stem Cells >> Extracellular
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Growth Factors/Hormones >> TGF
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Relevance
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BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily of secreted growth factors. It is a disulfide-linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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The antibody is provided in antibody stabilization buffer containing phosphate buffer, pH 7.4, glycerol, stabilizing proteins and preservatives. The exact buffer contents are considered proprietary.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BMP2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BMP2 antibody images, references, products related to ab14933 and other tools.
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BMP2 antibody images:
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 Immunohistochemistry (Frozen sections) - BMP2 antibody (ab14933)
ab14933 at 1/500 dilution staining human bone tissue sections by Immunohistochemistry (Frozen sections). The tissue was fixed with methanol and blocked with BSA prior to incubation with the antibody for 14 hours. StreptABComplex/HRP duet was used as the secondary antibody in this experiment.
In the image specific staining is shown in brown at the site of new bone formation. The blue color is due to toluidine background staining. This image is courtesy of an Abreview submitted by Mr Siim Suutre
See Abreview
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Immunocytochemistry/ Immunofluorescence - BMP2 antibody (ab14933)
ICC/IF image of Rat primary hepatocytes culture cells stained with ab14933. The cells were fixed with 4% PFA.and incubated in 5% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14933, 1µg/ml) and (ab15640) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 568 (red) at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
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Immunocytochemistry/ Immunofluorescence - BMP2 antibody (ab14933)
ab14933 at 1/200 dilution staining BMP2 in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG was used as secondary at 1/200 dilution.Cells were cultured in DMEM containing 10% FBS and Nuclei stained with DAPI.
See Abreview
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References for BMP2 antibody (ab14933)
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This product has been used in: (two most recent references)
Torihashi S et al. The expression and crucial roles of BMP signaling in development of smooth muscle progenitor cells in the mouse embryonic gut. Differentiation 77:277-89 (2009). PubMed: 19272526
Dobrowolski R et al. Loss of connexin43-mediated gap junctional coupling in the mesenchyme of limb buds leads to altered expression of morphogens in mice. Hum Mol Genet 18:2899-911 (2009). PubMed: 19439426
If you publish research using ab14933 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to BMP2
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BMP2 antibody - more information
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Customer reviews (feedback) regarding BMP2 antibody |
Customer FAQs regarding BMP2 antibody |
Protocols for BMP2 antibody |
Price and availability of products related to BMP2 antibody |
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BMP2 antibody - related products:
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BMP2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about BMP2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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