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Product Name
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BMP2 protein
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Product type
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Proteins and Peptides
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Peptide
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Recombinant Human BMP2. A 26 kDa homodimeric protein consisting of two 115 amino acid polypeptide chains.
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Tested applications
(see key)
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CellAct, SDS-PAGE
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Application notes
(see key)
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Recommended dilutions
CellAct: The ED50 for inducing alkaline phosphatase production by ATDC-5 cells is < 1µg/ml.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Stem Cells >> Stem Cell Cytokines >> Recombinant Human
Cancer >> Growth factors >> TGF
Stem Cells >> Neural Stem Cells >> Extracellular
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Growth Factors/Hormones >> TGF
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Relevance
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BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily of secreted growth factors. It is a disulfide-linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis.
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: None
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
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Purification notes
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Greater than 98% by SDS-PAGE.
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Form
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Lyophilised
Centrifuge the vial prior to opening. Reconstitute in water containing BSA (50ug of BSA per 1ug of protein) to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Expression system
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E. coli
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Sequence
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MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BMP2 protein is on this datasheet. But please do contact us if you would like any reassurance! |
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References for BMP2 protein (ab50099)
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This product has been used in: (two most recent references)
Wong CE et al. Neural crest-derived cells with stem cell features can be traced back to multiple lineages in the adult skin. J Cell Biol 175:1005-15 (2006). PubMed: 17158956
Chou YT & Yang YC Post-transcriptional control of Cited2 by transforming growth factor beta. Regulation via Smads and Cited2 coding region. J Biol Chem 281:18451-62 (2006). PubMed: 16675452
See all 4 publication references for this product.
If you publish research using ab50099 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to BMP2
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BMP2 protein - more information
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Customer reviews (feedback) regarding BMP2 protein |
Customer FAQs regarding BMP2 protein |
Protocols for BMP2 protein |
Price and availability of products related to BMP2 protein |
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BMP2 protein - other tools:
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Contact Abcam with a Technical Enquiry about BMP2 protein |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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