BMP4 antibody [66119.11] (ab6296)

Product Name 

BMP4 antibody [66119.11]

Product type 

Primary antibodies

Description 

Mouse monoclonal [66119.11] to BMP4

Immunogen 

Recombinant full length protein expressed in NSO cells (Human).

Reacts with 
(species key)

Hu

Specificity 

Monoclonal Anti-BMP-4 neutralizes human BMP-4, and recognizes recombinant human BMP-4 by ELISA and immunoblotting. The antibody shows approximately 5% cross-reactivity with recombinant human BMP-2, recombinant mouse BMP RIA and BMP-RIB.

Tested applications 
(see key)

I-ELISA, Neut, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
I-ELISA: Use at a concentration of 0.5 - 1.0 µg/ml detects a limit of around 6 ng/well of human BMP-4.
Neut: Use at a concentration of 1 - 3 µg/ml to neutralise 50% of the bioactivity due to 0.5 µg/ml recombinant human BMP-4
I-WB: Use at a concentration of 1 - 2 µg/ml (using human BMP-4 at 50 ng/lane under non-reducing and reducing conditions).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted, extracellular matrix.

Research areas 

Developmental Biology >> Organogenesis >> Excretory system development >> Kidney development
Developmental Biology >> Organogenesis >> Nervous system development
Developmental Biology >> Organogenesis >> Gut development >> Gut specification
Cancer >> Growth factors >> TGF
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Signal Transduction >> Growth Factors/Hormones >> TGF
Cardiovascular >> Angiogenesis >> Growth Factors >> TGF

Relevance 

BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide-linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. Smad1 translocation to the nucleus is observed after the addition of BMP4 (also designated BMP2B), suggesting that BMP4 may play a role in activation of the Smad pathway. BMP is secreted into the extracellular matrix.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

66119.11

Isotype 

IgG2b

Purity 

Protein G purified

Storage buffer 

n/a

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for BMP4 antibody [66119.11] (ab6296)

  This product has been used in:

Bull TM et al. Human Herpesvirus-8 Infection of Primary Pulmonary Microvascular Endothelial Cells. Am J Respir Cell Mol Biol : (2008). PubMed: 18587055

If you publish research using ab6296 please let us know so that we can cite the reference on this datasheet.

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