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RabMAbs (5163-1)

Anti-BMP4 antibody [EPR6211] (ab124715)

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Overview

  • Product nameAnti-BMP4 antibody [EPR6211]See all BMP4 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR6211] to BMP4
  • Tested applicationsWB, IP, IHC-P, Flow Cyt, ICC/IF more details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    A synthetic peptide corresponding to residues in Human BMP4

  • Positive controlHuman fetal liver, A549, HeLa, A431, and Human heart lysates; Human colon tissue.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab124715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/10000 - 1/50000. Predicted molecular weight: 47 kDa.
IP IP: 1/10 - 1/100.
IHC-P IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Flow Cyt: 1/100 - 1/500.
ICC/IF ICC/IF: 1/50 - 1/100.

Target

  • FunctionInduces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
  • Tissue specificityExpressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
  • Involvement in diseaseDefects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
    Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P12644 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        BMP 2B antibodyBMP 4 antibodyBMP-2B antibody
        BMP-4 antibodyBMP2B antibodyBMP2B1 antibodyBMP4 antibodyBMP4_HUMAN antibodyBone morphogenetic protein 2B antibodyBone morphogenetic protein 4 antibodyDVR4 antibodyMCOPS6 antibodyOFC11 antibodyZYME antibody
      see all

    Anti-BMP4 antibody [EPR6211] images

    • Western blot - Anti-BMP4 antibody [EPR6211] (ab124715)
      Western blot - Anti-BMP4 antibody [EPR6211] (ab124715)
      All lanes : Anti-BMP4 antibody [EPR6211] (ab124715) at 1/10000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : A549 lysate
      Lane 3 : HeLa lysate
      Lane 4 : A431 lysate
      Lane 5 : Human heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 47 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] (ab124715)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] (ab124715)
      ab124715, at 1/100, staining BMP4 in paraffin embedded Human colon tissue by Immunohistochemistry.
    • Other-Anti-BMP4 antibody [EPR6211](ab124715)
      Other-Anti-BMP4 antibody [EPR6211](ab124715)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-BMP4 antibody [EPR6211] (ab124715)

    ab124715 has not yet been referenced specifically in any publications.

    Publishing research using ab124715 ? Please let us know so that we can cite the reference in this datasheet

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