Anti-BMP4 antibody [EPR6211] (ab124715)
- Product nameAnti-BMP4 antibody [EPR6211]See all BMP4 primary antibodies ...
- DescriptionRabbit monoclonal [EPR6211] to BMP4
- Tested applicationsWB, IP, IHC-P, Flow Cyt, ICC/IF more details
- Species reactivityReacts with: Human
Does not react withMouse, Rat
A synthetic peptide corresponding to residues in Human BMP4
- Positive controlHuman fetal liver, A549, HeLa, A431, and Human heart lysates; Human colon tissue.
- General notesProduced under U.S. Patent No. 5,675,063.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Dissociation constant (Kd) Kd = 1.94 x 10-10 M
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR6211
Our Abpromise guarantee covers the use of ab124715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/10000 - 1/50000. Predicted molecular weight: 47 kDa.|
|IP||IP: 1/10 - 1/100.|
|IHC-P||IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||Flow Cyt: 1/100 - 1/500.|
|ICC/IF||ICC/IF: 1/50 - 1/100.|
- FunctionInduces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
- Tissue specificityExpressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
- Involvement in diseaseDefects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero.
- Sequence similaritiesBelongs to the TGF-beta family.
- Cellular localizationSecreted > extracellular space > extracellular matrix.
- BMP 2B antibodyBMP 4 antibodyBMP-2B antibody
- BMP-4 antibodyBMP2B antibodyBMP2B1 antibodyBMP4 antibodyBMP4_HUMAN antibodyBone morphogenetic protein 2B antibodyBone morphogenetic protein 4 antibodyDVR4 antibodyMCOPS6 antibodyOFC11 antibodyZYME antibody
Anti-BMP4 antibody [EPR6211] images
All lanes : Anti-BMP4 antibody [EPR6211] (ab124715) at 1/10000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : A549 lysate
Lane 3 : HeLa lysate
Lane 4 : A431 lysate
Lane 5 : Human heart lysate
Lysates/proteins at 10 µg per lane.
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 47 kDa
ab124715, at 1/100, staining BMP4 in paraffin embedded Human colon tissue by Immunohistochemistry.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
References for Anti-BMP4 antibody [EPR6211] (ab124715)
ab124715 has not yet been referenced specifically in any publications.