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ab40140 |
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ab40140 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab39973? Please let us know so that we can cite the reference in this datasheet
ab39973 has been referenced in 4 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-BMP4 antibody (ab39973) at 1 µg/ml
Lane 1 :
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Predicted band size : 47 kDa
Observed band size : 47 kDa
Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.
All lanes : Anti-BMP4 antibody (ab39973) at 1 µg/ml
Lane 1 :
Lane 2 : Brain (Mouse) Mouse Tissue Lysate
Lane 3 :
Lane 4 :
Lane 5 :
Lane 6 :
Lane 7 : Brain (Mouse) Mouse Tissue Lysate with
Lane 8 :
Lane 9 :
Lane 10 :
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 47 kDa
Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
ab39973 detects a 47kDa band that we believe corresponds to the pre-cleaved form of BMP4 with the immunizing peptide raised against a region located in the propeptide. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
All lanes : Anti-BMP4 antibody (ab39973) at 1/1000 dilution
Lane 1 : mouse brain
Lane 2 : mouse brain
Lane 3 : mouse brain
Lane 4 : mouse brain
Lysates/proteins at 20 µg per lane.
Secondary
Dako Cytomation P0260
Conjugation: HRP at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 47 kDa
Exposure time : 1 minute
Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
ICC/IF image of ab39973 staining primary hippocampal neurons. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39973, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
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