Anti-BMP4 antibody (ab39973)

ab58803 gave no bands in WB using positive control ab3871
ab39973 gave no bands in WB using positive control ab29466

ANSWER:

Thank you for contacting us.

As we discussed on the phone, you may be able to improve your WB results by switching from milk to BSA and by using a higher concentration of primary antibody (1:500 for example). If you are unable to improve your results, please let me know and I will be happy to offer you either replacements with alternative antibodies or credits on your original purchase. Please let me know how you would like to proceed and I will be happy to assist you further.

I would like to test ab39973 in IHC-Fronpig tissue. Could you please send information about the 100% Abreview discount?

ANSWER:

DISCOUNT CODE:


Expiration date:

I am very pleased to hear you would like to accept our offer and test ab39973 inIHC-Fr on pig. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forIHC-Fr onpigand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Dear Abcam. What is the homology of the imunogen and this ovine sequence, Accession: ABW37747.1 ? Kind regards

ANSWER:

Thank you very much for your interest in ab39973. I can confirm that this antibody has not yet been tested with sheep samples The immunogen shows 84% homology with the sequence you are interested in. We usually expect a cross-reaction for an antibody from 85% homology and up. I am aware that this homology is only boarder line, but would still like to offer a discount off a future purchase if you buy ab39973 now, test it in Ovis aries (sheep) and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab39973 in sheep. I will then send a discount code. This code must be issued before purchasing ab39973 so please wait for my reply before ordering. 2. Purchase ab39973 either by phone, fax, or online (www.abcam.com). 3. Test it in sheep. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab39973 turns out to be unsuitable for sheep, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Dear abcam,

Could the recombinant protein for human BMP4 (ab92855) be used as a positive control in western blot experiments for your Anti-BMP4 antibody (ab39973)? If not could you recommend another combination of positive control/antibody for application in western blot targetting BMP4.

Kind regards,

ANSWER:

Thank you for your enquiry.

The ab92855 BMP4 protein (MBP) and ab39973 BMP4 antibody have both been tested and guaranteed in western blot. The two products have not been specifically tested together in the laboratory, but as the protein is full length, it should be detected by the ab39973 antibody.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

What is the homology of the immunogen and the chicken sequence, accession NM_205237?

ANSWER:

Thank you for contacting us. The gallus BMP4 sequence shares 11 of 13 amino acids with the immunogen for ab39973: + ++++ ++++++ That may be sufficient for cross-reaction but the only species that have been tested, to our knowledge, are human mouse and rat. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.