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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> TGF
Overview
Product name
Anti-BMP4 antibody
See all BMP4 products (9) ...
Description
Rabbit polyclonal to BMP4
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow, Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BMP4.
(Peptide available as ab40140.)
Positive control
ab39973 gave a positive signal in the following Tissue Lysates: Brain (Human), Brain (Mouse), Liver (Human), Heart (Human) and Skeletal Muscle (Human).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Excretory system development >> Kidney development
Developmental Biology >> Organogenesis >> Nervous system development
Developmental Biology >> Organogenesis >> Gut development >> Gut specification
Cancer >> Growth factors >> TGF
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Signal Transduction >> Growth Factors/Hormones >> TGF
Cardiovascular >> Angiogenesis >> Growth Factors >> TGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).Can be blocked with BMP4 peptide (ab40140).
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
Tissue specificity
Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
Involvement in disease
Defects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero.
Sequence similarities
Belongs to the TGF-beta family.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP12644
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BMP 2B antibody
- BMP 4 antibody
- BMP-2B antibody
see all
Database links
- Entrez Gene: 407216 Cow
- Entrez Gene: 652 Human
- Entrez Gene: 12159 Mouse
- Entrez Gene: 25296 Rat
- Omim: 112262 Human
- SwissProt: Q2KJH1 Cow
- SwissProt: P12644 Human
- SwissProt: P21275 Mouse
see all
Anti-BMP4 antibody images:
Western blot - BMP4 antibody (ab39973)
All lanes : Anti-BMP4 antibody (ab39973) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Predicted band size : 47 kDa
Observed band size : 47 kDa
Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.
Western blot - BMP4 antibody (ab39973)
All lanes : Anti-BMP4 antibody (ab39973) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Brain (Mouse) Mouse Tissue Lysate
Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
Lane 4 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
Lane 5 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)
Lane 6 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466) with BMP4 peptide (ab40140) at 1 µg/ml
Lane 7 : Brain (Mouse) Mouse Tissue Lysate with BMP4 peptide (ab40140) at 1 µg/ml
Lane 8 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889) with BMP4 peptide (ab40140) at 1 µg/ml
Lane 9 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431) with BMP4 peptide (ab40140) at 1 µg/ml
Lane 10 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) with BMP4 peptide (ab40140) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 47 kDa
Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
ab39973 detects a 47kDa band that we believe corresponds to the pre-cleaved form of BMP4 with the immunizing peptide raised against a region located in the propeptide. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Western blot - BMP4 antibody (ab39973)
All lanes : Anti-BMP4 antibody (ab39973) at 1/1000 dilution
Lane 1 : mouse brain
Lane 2 : mouse brain
Lane 3 : mouse brain
Lane 4 : mouse brain
Lysates/proteins at 20 µg per lane.
Secondary
Dako Cytomation P0260
Conjugation: HRP at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 47 kDa
Exposure time : 1 minute
Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
Immunocytochemistry/ Immunofluorescence - BMP4 antibody (ab39973)
ICC/IF image of ab39973 staining primary hippocampal neurons. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39973, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
References for Anti-BMP4 antibody (ab39973)
This product has been referenced in:
- Ulyanova Tet al. Combinatorial and distinct roles of a5 and a4 integrins in stress erythropoiesis in mice. Blood 117:975-85 (2011). Mouse.Read more (PubMed: 20956802) »
- Rui YFet al. Expression of chondro-osteogenic BMPs in clinical samples of patellar tendinopathy. Knee Surg Sports Traumatol Arthrosc : (2011). IHC-P; Human.Read more (PubMed: 21946950) »
See all 3 publications for this product
Publishing research using ab39973? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"