Overview
- Product nameBMP5 293T Transfected Lysate - (positive control)
- General notesab94188 is a 293T cell transfected lysate in which Human BMP5 has been transiently over-expressed using a pCMV-BMP5 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
- Tested applicationsWB more details
Properties
- FormLiquid
- Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
- Storage bufferpH: 6.80
Constituent: 100% 1x Sample Buffer -
Concentration information loading... - PurityWhole Cell Lysate
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab94188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB |
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
BMP5 293T Transfected Lysate - (positive control) images
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SDS-PAGE - BMP5 293T Transfected Lysate - (positive control) (ab94188)ab94188 at 15 µg/lane on an SDS-PAGE gel -
Western blot - BMP5 293T Transfected Lysate - (positive control) (ab94188)All lanes : Anti-BMP5 antibody (ab88064) at 1/500 dilution
Lane 1 :BMP5 293T Transfected Lysate - (positive control) (ab94188)
Lane 2 : 293T non-transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
Protocols
References for BMP5 293T Transfected Lysate - (positive control) (ab94188)
ab94188 has not yet been referenced specifically in any publications.
