![Western blot - BMP5 antibody [1G6] (ab88064) image](#BMP5-Primary-antibodies-ab88064-1.jpg)
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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> TGF
Overview
Product name
Anti-BMP5 antibody
See all BMP5 products (5) ...
Description
Mouse monoclonal to BMP5
Tested applications
ICC/IF, WB, ELISAmore details
Cross reactivity
Reacts with
Rat, Human, Recombinant Fragment
Immunogen
Recombinant fragment, corresponding to amino acids 323-454 of Human BMP5 with proprietary tag (NP_066551)
Positive control
Hela cells Nuclear extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Growth Factors/Hormones >> TGF
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
Cardiovascular >> Angiogenesis >> Growth Factors >> TGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab88064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 52 kDa.
ELISA: Use at an assay dependent dilution.
Target
Function
Induces cartilage and bone formation.
Tissue specificity
Expressed in the lung and liver.
Sequence similarities
Belongs to the TGF-beta family.
Cellular localization
Secreted.
Target information above from: UniProt accessionP22003
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BMP 5 antibody
- BMP-5 antibody
- Bmp5 antibody
see all
Database links
- Entrez Gene: 653 Human
- Entrez Gene: 315824 Rat
- Omim: 112265 Human
- SwissProt: P22003 Human
- Unigene: 296648 Human
- Unigene: 135799 Rat
Anti-BMP5 antibody images:
Western blot - BMP5 antibody [1G6] (ab88064)
![Western blot - BMP5 antibody [1G6] (ab88064)](/ps/datasheet/images/88/ab88064/BMP5-Primary-antibodies-ab88064-1.jpg)
Anti-BMP5 antibody (ab88064) at 5 µg/ml + HeLa cells nuclear extract at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 52 kDa
Observed band size : 52 kDa
Immunocytochemistry/ Immunofluorescence - Anti-BMP5 antibody (ab88064)
ICC/IF image of ab88064 staining of BMP5 in rat hippocampal culture. The sections were incubated in 0.3% triton X to permeabilise the cells and then in 10% serum to block non-specific protein-protein interactions. A two hour incubation at +24°C was performed with ab88064 (1:1000), followed by Alexa Fluor 568 conjugated secondary antibody. Red staining of the hippocampal neurons was observed.
This image was taken from an abreview submitted by an Ruma Raha-Chowdhury.
ELISA - BMP5 antibody (ab88064)
Detection limit for recombinant tagged BMP5 is approximately 1ng/ml as a capture antibody.
References for Anti-BMP5 antibody (ab88064)
ab88064 has not yet been referenced specifically in any publications.
Publishing research using ab88064? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"