BMP6 Human ELISA kit (ab99984)
- Product nameBMP6 Human ELISA kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 150 pg/ml
- Range200 pg/ml - 20000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 93.88 84% - 107% Serum 96.53 85% - 104% Plasma 94.69 84% - 103%
- Species reactivityReacts with: Human
- Product overview
ab99984 Human BMP6 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BMP6 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human BMP6 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 8 strips 12 units Assay Diluent 5x concentrated buffer. For standard/sample (serum/plasma samples/cell culture medium/urine) and reagent diluent 1 x 15ml BMP 6 Microplate (12 strips x 8 wells) coated with anti-human BMP-6 1 unit BMP 6 Standard Human (recombinant) 2 vials Detection Antibody BMP-6 biotinylated anti-human BMP-6 2 vials HRP-Streptavidin Concentrate 1500x 1 x 200µl Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- BMP 6Bone morphogenetic protein 6Vegetal related (TGFB related) cytokine
- Vegetal related growth factor (TGFB related)VG 1 RVG 1 related proteinVg1 related sequenceVGRVGR 1VGR1
Our Abpromise guarantee covers the use of ab99984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for BMP6 Human ELISA kit (ab99984)
ab99984 has not yet been referenced specifically in any publications.