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Products:Signal Transduction >> Growth Factors/Hormones >> TGF
Anti-BMP6 antibody [morph-6.1] (ab15640)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab15640 for help.
There are no answered questions for ab15640
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunocytochemistry/ Immunofluorescence - BMP6 antibody [morph-6.1] (ab15640)](/ps/datasheet/Images/15/ab15640/ab15640_review_12693.jpg)
Immunocytochemistry/ Immunofluorescence - BMP6 antibody [morph-6.1] (ab15640)
ab15640 staining human U2-OS cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton-X-100 prior to blocking in 2% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 37°C. The secondary antibody was Alexa Fluor®488 conjugated chicken anti-mouse, diluted 1/1000.
This image is courtesy of an anonymous Abreview
![Western blot - BMP6 antibody [morph-6.1] (ab15640)](/ps/datasheet/images/15/ab15640/BMP6-Primary-antibodies-ab15640-2.jpg)
Western blot - BMP6 antibody [morph-6.1] (ab15640)
All lanes : Anti-BMP6 antibody [morph-6.1] (ab15640) at 1/400 dilution
Lane 1 : mouse liver
Lane 2 : mouse liver
Lane 3 : mouse liver
Lane 4 : mouse liver
Lysates/proteins at 20 µg per lane.
Secondary
Non-Abcam Antibody was used: Dako Cytomation P0260
Conjugation: HRP at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 57 kDa
Observed band size : 57 kDa
Additional bands at : 25&30&75 kDa (possible non-specific binding).
Exposure time : 10 seconds
This image is courtesy of an Abreview submitted by Dr Ruma Raha-Chowdhury
![Immunocytochemistry/ Immunofluorescence - BMP6 antibody [morph-6.1] (ab15640)](/ps/datasheet/images/15/ab15640/BMP6-Primary-antibodies-ab15640-4.jpg)
Immunocytochemistry/ Immunofluorescence - BMP6 antibody [morph-6.1] (ab15640)
ICC/IF image of ab15640 staining primary myoblast cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15640, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h.
Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
![BMP6 antibody [morph-6.1] for ICC/IF in Rat (15640)](/ps/datasheet/images/15/ab15640/BMP6-Primary-antibodies-ab15640-3.jpg)
BMP6 antibody [morph-6.1] for ICC/IF in Rat (15640)
![BMP6 antibody [morph-6.1] for WB in Rat (15640)](/ps/Reviews/Images/ab9946_51006.jpg)
BMP6 antibody [morph-6.1] for WB in Rat (15640)
![BMP6 antibody [morph-6.1] for WB in Mouse (15640)](/ps/datasheet/images/15/ab15640/BMP6-Primary-antibodies-ab15640-1.jpg)
BMP6 antibody [morph-6.1] for WB in Mouse (15640)
![BMP6 antibody [morph-6.1] for ICC/IF in Human (15640)](/ps/Reviews/Images/ab12693_70906.jpg)
BMP6 antibody [morph-6.1] for ICC/IF in Human (15640)
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