|
|
|
|
Immunocytochemistry/ Immunofluorescence - BMP6 antibody [morph-6.1] (ab15640)
![Immunocytochemistry/ Immunofluorescence - BMP6 antibody [morph-6.1] (ab15640)](/ps/datasheet/images/ab15640_review_12693.jpg)
(enlarge)
|
|
Western blot - BMP6 antibody [morph-6.1] (ab15640)
![Western blot - BMP6 antibody [morph-6.1] (ab15640)](/ps/datasheet/images/15/ab15640/BMP6-Primary-antibodies-ab15640-2.jpg)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence - BMP6 antibody [morph-6.1] (ab15640)
![Immunocytochemistry/ Immunofluorescence - BMP6 antibody [morph-6.1] (ab15640)](/ps/datasheet/images/15/ab15640/BMP6-Primary-antibodies-ab15640-4.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
BMP6 antibody [morph-6.1]
|
| |
See all BMP6 antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [morph-6.1] to BMP6
|
|
Immunogen
|
Synthetic peptide: MPGLGRRAQWLCWWWGLLCSCCGPPPLRP conjugated to KLH, corresponding to amino acids 1-29 of Rat BMP6.
BLAST 'MPGLGRRAQWLCWWWGLLCSCCGPPPLRP' with  or 
|
|
Reacts with
(species key)
|
Hu, Ms, Rat, Dog
|
|
Tested applications
(see key)
|
ICC/IF, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB: 1/100. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Positive staining can be observed in embryonic and adult central nervous system. Rat radial glial cells of the developing central nervous system from E11 to E19. In rat peripheral nerves a selective intracellular immunoreactivity can be found in perinuclear region of most Schwann cells which form the myelin sheath. However, some Schwann cells were negative for the antigen.
|
|
Cellular localization
|
Secreted
|
|
Research areas
|
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Growth Factors/Hormones >> TGF
|
|
Relevance
|
Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species. BMPs are involved in embryogenesis and morphogenesis of various tissues and organs. BMP6 is an autocrine stimulator of chondrocyte differentiation and has been implicated in the development of embryonic kidney and urinary systems. It is involved in liver growth and differentiation, keratinocyte differentiation, and regulation of neuronal tissue development. BMP6 expression is localized to smooth muscle cells of normal cerebral blood vessels, intimal plaques and atherosclerotically changed blood vessels, further suggesting a role as a regulator of developing tissues.
Pre-incubation of anti BMP6 with amino-terminal peptide 1-29 of BMP6 inhibits binding of the mAb to tissue sections. Pre-incubation with other amino-terminal synthetic peptides of human BMP7, BMP4, BMP3 and BMP2 as well as an irrelevant peptide does not inhibit specific tissue staining.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
morph-6.1
|
|
Isotype
|
IgG1
|
|
Purity
|
IgG fraction
|
|
Storage buffer
|
Preservative: 0.01% Thimerosal (merthiolate)
Material safety datasheet (MSDS) for this product:
Thimerosal MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.200 mg/ml
|
|
Storage instructions
|
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BMP6 antibody [morph-6.1] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for BMP6 antibody [morph-6.1] images, references, products related to ab15640 and other tools.
|
| |
BMP6 antibody [morph-6.1] images:
|
 Immunocytochemistry/ Immunofluorescence - BMP6 antibody [morph-6.1] (ab15640)
ab15640 staining human U2-OS cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton-X-100 prior to blocking in 2% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 37°C. The secondary antibody was Alexa Fluor®488 conjugated chicken anti-mouse, diluted 1/1000. This image is courtesy of an anonymous Abreview
See Abreview
|
| |
Western blot - BMP6 antibody [morph-6.1] (ab15640)
All lanes : BMP6 antibody [morph-6.1] (ab15640) at 1/400 dilution
Lane 1 : mouse liver
Lane 2 : mouse liver
Lane 3 : mouse liver
Lane 4 : mouse liver
Lysates/proteins at 20 µg per lane.
Secondary
Non-Abcam Antibody was used: Dako Cytomation P0260
Conjugation: HRP at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 57 kDa
Observed band size : 57 kDa
Additional bands at : 25&30&75 kDa (possible non-specific binding).
Exposure time : 10 seconds This image is courtesy of an Abreview submitted by Dr Ruma Raha-Chowdhury
See Abreview
|
| |
Immunocytochemistry/ Immunofluorescence - BMP6 antibody [morph-6.1] (ab15640)
ICC/IF image of ab15640 staining primary myoblast cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15640, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
|
| |
References for BMP6 antibody [morph-6.1] (ab15640)
|
|
This product has been used in: (two most recent references)
Wensman H et al. Diverse bone morphogenetic protein expression profiles and smad pathway activation in different phenotypes of experimental canine mammary tumors. PLoS One 4:e7133 (2009). IHC-P; Dog. PubMed: 19771160
Jung Y et al. Hematopoietic stem cells regulate mesenchymal stromal cell induction into osteoblasts thereby participating in the formation of the stem cell niche. Stem Cells 26:2042-51 (2008). IHC-P; Mouse. PubMed: 18499897
If you publish research using ab15640 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to BMP6
|
| |
BMP6 antibody [morph-6.1] - more information
|
Customer reviews (feedback) regarding BMP6 antibody [morph-6.1] |
Customer FAQs regarding BMP6 antibody [morph-6.1] |
Protocols for BMP6 antibody [morph-6.1] |
Price and availability of products related to BMP6 antibody [morph-6.1] |
| |
BMP6 antibody [morph-6.1] - related products:
|
|
|
| |
BMP6 antibody [morph-6.1] - other tools:
|
Contact Abcam with a Technical Enquiry about BMP6 antibody [morph-6.1] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunocytochemistry/ Immunofluorescence - BMP6 antibody [morph-6.1] (ab15640)](/index.html?datasheet=15640#ab15640_review_12693.jpg)
![Western blot - BMP6 antibody [morph-6.1] (ab15640)](/index.html?datasheet=15640#BMP6-Primary-antibodies-ab15640-2.jpg)
![Immunocytochemistry/ Immunofluorescence - BMP6 antibody [morph-6.1] (ab15640)](/index.html?datasheet=15640#BMP6-Primary-antibodies-ab15640-4.jpg)
