Anti-BMP6 antibody [morph-6.1] (ab15640)

  Immunocytochemistry/ Immunofluorescence - BMP6 antibody [morph-6.1] (ab15640)

ab15640 staining human U2-OS cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton-X-100 prior to blocking in 2% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 37°C. The secondary antibody was Alexa Fluor®488 conjugated chicken anti-mouse, diluted 1/1000.

This image is courtesy of an anonymous Abreview

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  Western blot - BMP6 antibody [morph-6.1] (ab15640)

All lanes : Anti-BMP6 antibody [morph-6.1] (ab15640) at 1/400 dilution

Lane 1 : mouse liver
Lane 2 : mouse liver
Lane 3 : mouse liver
Lane 4 : mouse liver

Lysates/proteins at 20 µg per lane.

Secondary
Non-Abcam Antibody was used: Dako Cytomation P0260 Conjugation: HRP at 1/3000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 57 kDa
Observed band size : 57 kDa
Additional bands at : 25&30&75 kDa (possible non-specific binding).

Exposure time : 10 seconds

This image is courtesy of an Abreview submitted by Dr Ruma Raha-Chowdhury

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  Immunocytochemistry/ Immunofluorescence - BMP6 antibody [morph-6.1] (ab15640)

ICC/IF image of ab15640 staining primary myoblast cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15640, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h.

Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom