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BMP7 293T Transfected Lysate - (positive control) (ab94187)

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Overview

  • Product nameBMP7 293T Transfected Lysate - (positive control)
  • General notesab94187 is a 293T cell transfected lysate in which Human BMP7 has been transiently over-expressed using a pCMV-BMP7 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Tissue specificity: Expressed in the kidney and bladder. Lower levels seen in the brain. Similarity: Belongs to the TGF-beta family. Developmental stage: Expressed in the developing eye, brain and ear during embryogenesis. PTM: Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).

    • Applications

    Our Abpromise guarantee covers the use of ab94187 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • BMP7 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - BMP7 293T Transfected Lysate - (positive control) (ab94187)
      SDS-PAGE - BMP7 293T Transfected Lysate - (positive control) (ab94187)
      ab94187 at 15 µg/lane on an SDS-PAGE gel
    • Western blot - BMP7 293T Transfected Lysate - (positive control) (ab94187)
      Western blot - BMP7 293T Transfected Lysate - (positive control) (ab94187)
      All lanes : Anti-BMP7 antibody (ab54904) at 1/500 dilution

      Lane 1 : BMP7 293T Transfected Lysate - (positive control) (ab94187)
      Lane 2 : 293T non-transfected Lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for BMP7 293T Transfected Lysate - (positive control) (ab94187)

    ab94187 has not yet been referenced specifically in any publications.

    Publishing research using ab94187 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"