Anti-BMP7 antibody (ab27569)

  Immunocytochemistry/ Immunofluorescence - BMP7 antibody (ab27569)

ICC/IF image of ab27569 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27569, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Immunohistochemistry (PFA perfusion fixed frozen sections) - BMP7 antibody (ab27569)

ICC/IF image of Rat primary hepatocytes culture cells stained with ab27569. The cells were fixed with 4% PFA and incubated in  5% normal donkey serum in 0.1% PBS- and triton X100  for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27569, 1µg/ml) and (ab15640) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 568 (red) at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom