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Immunocytochemistry/ Immunofluorescence - BMP7 antibody (ab27569)
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Immunohistochemistry (PFA perfusion fixed frozen sections) - BMP7 antibody (ab27569)
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Product Name
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BMP7 antibody
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See all BMP7 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to BMP7
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Immunogen
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Recombinant full length human BMP7 protein.
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Reacts with
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Hu, Rat
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Tested applications
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ELISA, ICC/IF, Neut, WB, sELISA
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at a concentration of 0.5 µg/ml.
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
Neut: Use at an assay dependent dilution.
sELISA: Use at a concentration of 0.5 µg/ml. The complementary detection antibody is ab84030
WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 49 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Developmental Biology >> Organogenesis >> Excretory system development >> Kidney development
Developmental Biology >> Organogenesis >> Nervous system development
Cancer >> Cell cycle >> Cell differentiation
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Growth Factors/Hormones >> TGF
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Relevance
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Bone Morphogenetic Protein 7 (BMP7), also known as osteogenic protein 1 (OP1), is produced from a DNA sequence encoding the human BMP2 signal peptide and human BMP2 propeptide (amino acid residues 1 to 282) fused to the human BMP7 mature subunit (amino acid residues 293 to 431). Mature human BMP7, generated after the proteolytic removal of the signal peptide and the propeptide, is a disulfide linked homodimeric protein, comprised of two 139 amino acid residue subunits. Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents:0.5 X PBS, pH 7.4.
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Purification notes
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This antibody was purified by affinity chromatography employing immobilized hBMP7 matrix.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BMP7 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BMP7 antibody images, references, products related to ab27569 and other tools.
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BMP7 antibody images:
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 Immunocytochemistry/ Immunofluorescence - BMP7 antibody (ab27569)
ICC/IF image of ab27569 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27569, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - BMP7 antibody (ab27569)
ICC/IF image of Rat primary hepatocytes culture cells stained with ab27569. The cells were fixed with 4% PFA and incubated in 5% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27569, 1µg/ml) and (ab15640) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 568 (red) at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
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References for BMP7 antibody (ab27569)
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This product has been used in: (two most recent references)
Roxburgh SA et al. Allelic depletion of grem1 attenuates diabetic kidney disease. Diabetes 58:1641-50 (2009). WB; Human, Mouse. PubMed: 19401426
Yan W & Chen X Targeted repression of bone morphogenetic protein 7, a novel target of the p53 family, triggers proliferative defect in p53-deficient breast cancer cells. Cancer Res 67:9117-24 (2007). PubMed: 17909016
If you publish research using ab27569 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to BMP7
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BMP7 antibody - more information
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Customer reviews (feedback) regarding BMP7 antibody |
Customer FAQs regarding BMP7 antibody |
Protocols for BMP7 antibody |
Price and availability of products related to BMP7 antibody |
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BMP7 antibody - related products:
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BMP7 antibody - other tools:
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Contact Abcam with a Technical Enquiry about BMP7 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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