Anti-BMP7 antibody (ab56023)

Immunohistochemistry (Paraffin-embedded sections) - BMP7 antibody (ab56023)

ab56023, at a dilution of 4µg/ml, staining BMP7 in paraffin embedded human epithelial renal tubule cells by Immunohistochemistry.

Western blot - BMP7 antibody (ab56023)

Lane 1 : Anti-BMP7 antibody (ab56023) at 1.25 µg/ml
Lane 2 : Anti-BMP7 antibody (ab56023) at 2.5 µg/ml
Lane 3 : Anti-BMP7 antibody (ab56023) at 5 µg/ml

Lane 1 : Cell lysate prepared from BMP7 transfected 293T cells
Lane 2 : Cell lysate prepared from BMP7 transfected 293T cells
Lane 3 : Cell lysate prepared from BMP7 transfected 293T cells

Lysates/proteins at 25 µg per lane.


Predicted band size : 49 kDa

Immunocytochemistry/ Immunofluorescence - BMP7 antibody (ab56023)

ICC/IF image of ab56023 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56023, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Western blot - BMP7 antibody (ab56023)

All lanes : Anti-BMP7 antibody (ab56023) at 1/500 dilution

Lane 1 : Rat Brain Whole Tissue (age P8) Lysate
Lane 2 : Rat Brain Whole Tissue (age P15) Lysate
Lane 3 : Rat Brain Whole Tissue (age P21) Lysate
Lane 4 : Rat Brain Whole Tissue (age P21) Lysate

Lysates/proteins at 20 µg per lane.

Secondary
HRP conjugated at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 49 kDa
Observed band size : 49 kDa


Exposure time : 30 seconds

A number of additional non-specific bands were also observed at 17, 33, 35 & 40kDa

Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

Immunohistochemistry (Frozen sections) - BMP7 antibody (ab56023)

ab56023 staining mouse e18 skeleton tissues by immunohistochemistry (frozen sections). Sections were formaldehyde fixed prior to being blocked in 20% serum for 1 hour at RT. Sample was incubated with ab56023, diluted 1/1000, for 16 hours at 4ºC. Alexa fluor® 680 donkey polyclonal, diluted 1/200, was used as the secondary antibody.

This image is courtesy of an anonymous Abreview