Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Growth Factors/Hormones >> TGF
Overview
Product name
Anti-BMP7 antibody
See all BMP7 products (10) ...
Description
Rabbit polyclonal to BMP7
Specificity
This antibody reacts with BMP7.
Tested applications
IHC-Fr, WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Sheep, Cow, Dog, Pig
Immunogen
Synthetic peptide: QGKHNSAPMF MLDLYNAMAV EEGGGPGGQG FSYPYKAVFS TQGPPLASLQ, corresponding to N terminal amino acids 73-122 of Human BMP7. This sequence is derived from the propeptide domain, thus, will not recognize mature BMP7.
QGKHNSAPMF MLDLYNAMAV EEGGGPGGQG FSYPYKAVFS TQGPPLASLQ
Positive control
WB: 293T cell lysate. IHC-P: human kidney tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Excretory system development >> Kidney development
Developmental Biology >> Organogenesis >> Nervous system development
Cancer >> Cell cycle >> Cell differentiation
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Growth Factors/Hormones >> TGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
IHC-P: Use a concentration of 4 - 8 µg/ml.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
Target
Function
Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
Tissue specificity
Expressed in the kidney and bladder. Lower levels seen in the brain.
Sequence similarities
Belongs to the TGF-beta family.
Developmental stage
Expressed in the developing eye, brain and ear during embryogenesis.
Post-translational
modifications
Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).
Cellular localization
Secreted.
Target information above from: UniProt accessionP18075
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BMP 7 antibody
- BMP-7 antibody
- Bmp7 antibody
see all
Database links
- Entrez Gene: 477270 Dog
- Entrez Gene: 655 Human
- Entrez Gene: 12162 Mouse
- Entrez Gene: 492315 Pig
- Entrez Gene: 85272 Rat
- Omim: 112267 Human
- SwissProt: P18075 Human
- SwissProt: P23359 Mouse
see all
Anti-BMP7 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - BMP7 antibody (ab56023)
ab56023, at a dilution of 4µg/ml, staining BMP7 in paraffin embedded human epithelial renal tubule cells by Immunohistochemistry.
Western blot - BMP7 antibody (ab56023)
Lane 1 : Anti-BMP7 antibody (ab56023) at 1.25 µg/ml
Lane 2 : Anti-BMP7 antibody (ab56023) at 2.5 µg/ml
Lane 3 : Anti-BMP7 antibody (ab56023) at 5 µg/ml
Lane 1 : Cell lysate prepared from BMP7 transfected 293T cells
Lane 2 : Cell lysate prepared from BMP7 transfected 293T cells
Lane 3 : Cell lysate prepared from BMP7 transfected 293T cells
Lysates/proteins at 25 µg per lane.
Predicted band size : 49 kDa
Immunocytochemistry/ Immunofluorescence - BMP7 antibody (ab56023)
ICC/IF image of ab56023 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56023, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - BMP7 antibody (ab56023)
All lanes : Anti-BMP7 antibody (ab56023) at 1/500 dilution
Lane 1 : Rat Brain Whole Tissue (age P8) Lysate
Lane 2 : Rat Brain Whole Tissue (age P15) Lysate
Lane 3 : Rat Brain Whole Tissue (age P21) Lysate
Lane 4 : Rat Brain Whole Tissue (age P21) Lysate
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 49 kDa
Observed band size : 49 kDa
Exposure time : 30 seconds
A number of additional non-specific bands were also observed at 17, 33, 35 & 40kDa
Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
Immunohistochemistry (Frozen sections) - BMP7 antibody (ab56023)
ab56023 staining mouse e18 skeleton tissues by immunohistochemistry (frozen sections). Sections were formaldehyde fixed prior to being blocked in 20% serum for 1 hour at RT. Sample was incubated with ab56023, diluted 1/1000, for 16 hours at 4°C. Alexa fluor® 680 donkey polyclonal, diluted 1/200, was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-BMP7 antibody (ab56023)
This product has been referenced in:
- Fiaschetti Get al. Bone morphogenetic protein-7 is a MYC target with prosurvival functions in childhood medulloblastoma. Oncogene 30:2823-35 (2011). ICC/IF; Human.Read more (PubMed: 21317922) »
- Filion TMet al. Elastomeric osteoconductive synthetic scaffolds with acquired osteoinductivity expedite the repair of critical femoral defects in rats. Tissue Eng Part A 17:503-11 (2011). IHC-P; Rat.Read more (PubMed: 20818999) »
See all 6 publications for this product
Publishing research using ab56023? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"