Anti-BMP7 antibody (ab84684)

Overview

• Product nameAnti-BMP7 antibodySee all BMP7 primary antibodies ...
• Description
  Rabbit polyclonal to BMP7
• Tested applicationsWB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  Predicted to work with: Rabbit, Horse, Chicken, Pig
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human BMP7.

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in both Human and Rat Brain Tissue Lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Developmental Biology
  • Organogenesis
  • Nervous system development

  • Developmental Biology
  • Organogenesis
  • Excretory system development
  • Kidney development

  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Secreted

  • Cancer
  • Cell cycle
  • Cell differentiation

  • Signal Transduction
  • Growth Factors/Hormones
  • TGF

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • Structures
  • Bone

  • Cell Biology
  • Cell Cycle
  • Cell differentiation

Applications

Target

• FunctionInduces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
• Tissue specificityExpressed in the kidney and bladder. Lower levels seen in the brain.
• Sequence similaritiesBelongs to the TGF-beta family.
• Developmental stageExpressed in the developing eye, brain and ear during embryogenesis.
• Post-translational
  modificationsSeveral N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).
• Cellular localizationSecreted.

Target information above from: UniProt accession P18075 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 395494 Chicken
  • Entrez Gene: 655 Human
  • Entrez Gene: 12162 Mouse
  • Entrez Gene: 492315 Pig
  • Entrez Gene: 85272 Rat
  • Omim: 112267 Human
  • SwissProt: P18075 Human
  • SwissProt: P23359 Mouse

  • Unigene: 473163 Human
  • Unigene: 595 Mouse
  • Unigene: 18030 Rat

  see all

• Alternative names
  BMP 7 antibodyBMP-7 antibodyBmp7 antibody

  BMP7_HUMAN antibodyBone morphogenetic protein 7 antibodyBone morphogenic protein 7 antibodyEptotermin alfa antibodyOP 1 antibodyOP-1 antibodyOP1 antibodyOsteogenic protein 1 antibody

  see all

Anti-BMP7 antibody images

• 

  Western blot - BMP7 antibody (ab84684)
  All lanes : Anti-BMP7 antibody (ab84684) at 1 µg/ml
  
  Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
  Lane 2 : Brain (Mouse) Tissue Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
  
  Predicted band size : 49 kDa
  Observed band size : 49 kDa
  Additional bands at : 10 kDa,33 kDa,38 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 30 seconds
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BMP7 antibody (ab84684)
  IHC image of BMP7 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84684, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
• 

  Western blot - Anti-BMP7 antibody (ab84684)
  Anti-BMP7 antibody (ab84684) at 1/1 dilution + BMP7 protein (Human) (ab83921) at 0.1 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time : 1 minute
• 

  Western blot - Anti-BMP7 antibody (ab84684)
  Anti-BMP7 antibody (ab84684) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µg
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 49 kDa
  Observed band size : 49 kDa
  Additional bands at : 37 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 8 minutes