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Products:Signal Transduction >> Growth Factors/Hormones >> TGF
Overview
Product name
Anti-BMPER antibody
See all BMPER products (2) ...
Description
Rabbit polyclonal to BMPER
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat, Chicken, Cow, Dog, Human, Chimpanzee, Rhesus monkey
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Mouse BMPER.
(Peptide available as ab74993.)
Positive control
This antibody gave a positive signal in the following Mouse Whole Cell Lysates: F9, NIH 3T3, EBS E14 TG2A Day 0
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Vasculature >> Endothelium
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Growth Factors/Hormones >> TGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab73900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 75,110 kDa (predicted molecular weight: 76 kDa).
IHC-P: 1/250Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Relevance
BMPER is a BMP-binding protein that is expressed by endothelial cell precursors, has BMP-antagonizing activity, and may play a role in endothelial cell differentiation by modulating local BMP activity. It may also regulate BMP responsiveness in osteoblasts and chondrocytes.
Cellular localization
Secreted
Alternative names
- BMP binding endothelial regulator antibody
- BMP binding endothelial regulator precursor protein antibody
- Bone morphogenetic protein binding endothelial cell precursor derived regulator antibody
see all
Database links
- Entrez Gene: 168667 Human
- Entrez Gene: 73230 Mouse
- Entrez Gene: 300455 Rat
- Omim: 608699 Human
- SwissProt: Q8N8U9 Human
- SwissProt: Q8CJ69 Mouse
Anti-BMPER antibody images:
Western blot - BMPER antibody (ab73900)
All lanes : Anti-BMPER antibody (ab73900) at 1 µg/ml
Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : EBS E14 TG2A Day 0 (Mouse Pluripotent Embryonic Stem Cell) Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 76 kDa
Observed band size : 75,110 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
BMPER has a predicted molecular weight of 76 kDa, however it is extensively glycosylated and has been seen to run in the 100 kDa region (other commercially available antibodies).
Immunocytochemistry/ Immunofluorescence - Anti-BMPER antibody (ab73900)
ICC/IF image of ab73900 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73900, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-BMPER antibody (ab73900)
ab73900 has not yet been referenced specifically in any publications.
Publishing research using ab73900? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"