BMPR1A antibody (ab10861)

Product Name 

BMPR1A antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to BMPR1A

Immunogen 

Recombinant full length protein (Human) expressed in mouse NSO cells.

Reacts with 
(species key)

Hu

Specificity 

Based on immunoblotting, this antibody exhibits no cross-reactivity with recombinant human BMP2, BMP4, BMP5, BMP6, or BMP7.

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 0.5 - 1.0 µg/ml.The detection limit for recombinant human BMP-5 is approximately 1.2 ng/well.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant human BMPR-IA is approximately 20 ng/lane under non-reducing and reducing conditions.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane

Research areas 

Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Signal Transduction >> Metabolism >> Vitamins / Minerals
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Stem Cells >> Signaling Pathways >> TGF beta >> Surface Molecules
Signal Transduction >> Growth Factors/Hormones >> TGF
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases

Relevance 

The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding.

Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species. Mature BMPs are 30-38 kDa proteins that assume a TGF beta-like cysteine knot configuration. Unlike TGF beta, BMPs do not form latent complexes with their propeptide counterparts. Most BMPs are homodimers, but bioactive natural heterodimers have been reported. Recently it was found that lovostatin, widely used for lowering cholesterol, also increases bone formation by turning on a gene (bmp2) that promotes local bone formation. BMPs are involved in embryogenesis andmorphogenesis of various tissues and organs. They create an environment conducive for bone marrow development by stimulating the production of specific bone matrix proteins and altering stromal cell and osteoclast proliferation. In addition to stimulating ectopic bone and cartilage development, BMPs may be an important factor in the development of the viscera. BMPs regulate the growth, differentiation, chemotaxis, proliferation, and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. BMPs also appear to be responsible for normal dorsal/ventral patterning and can found in tissues that induce bone or cartilage growth, such as demineralized bone and urinary epithelium. Cellular responses to BMPs are mediated by the formation of hetero-oligomeric complexes of the type and type II serine/threonine kinase receptors which play significant roles in BMP binding and signaling. Bone Morphogenetic Protein Receptor IA (BMPRIA), also known as activin receptor-like kinase ALK3, is type I serine/threonine kinase required for the signal transduction of the TGF beta family cytokines. BMP receptors include the type I receptors, BMPR1A and BMPR1B (50-55 kDa), and the type II receptor BMPRII (70-80 kDa). These receptors are also closely related to the activin receptors ACV R1 and ACV R2. Type I receptors involved in BMP signaling can independently bind the various BMP family proteins in the absence of type II receptors. Soluble BMPRIA binds BMP4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. In adult tissues, BMPRIA is widely expressed, but the highest expression levels are found in skeletal muscle. BMPRIA is also widely expressed during embryogenesis.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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