Overview
- Product nameAnti-BMPR1A antibodySee all BMPR1A primary antibodies ...
- DescriptionRabbit polyclonal to BMPR1A
- Tested applicationsWB, ELISA, IHC-P more details
- Species reactivityReacts with: Human
- Immunogen
Synthetic peptide (10-30 aa in length) from the N-terminus of human BMPR1A, conjugated to KLH.
- Positive controlHuman breast carcinoma tissue.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityProtein G purified
- Purification notesab59945 is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab59945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
| ELISA | ELISA: 1/1000. |
| IHC-P | IHC-P: 1/50 - 1/100. |
Target
- FunctionOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP-2 and BMP-4.
- Tissue specificityHighly expressed in skeletal muscle.
- Involvement in diseaseDefects in BMPR1A are a cause of juvenile polyposis syndrome (JPS) [MIM:174900]; also known as juvenile intestinal polyposis (JIP). JPS is an autosomal dominant gastrointestinal hamartomatous polyposis syndrome in which patients are at risk for developing gastrointestinal cancers. The lesions are typified by a smooth histological appearance, predominant stroma, cystic spaces and lack of a smooth muscle core. Multiple juvenile polyps usually occur in a number of Mendelian disorders. Sometimes, these polyps occur without associated features as in JPS; here, polyps tend to occur in the large bowel and are associated with an increased risk of colon and other gastrointestinal cancers.
Defects in BMPR1A are a cause of Cowden disease (CD) [MIM:158350]. CD is an autosomal dominant cancer syndrome characterized by multiple hamartomas and by a high risk for breast, thyroid and endometrial cancers.
Defects in BMPR1A are the cause of hereditary mixed polyposis syndrome 2 (HMPS2) [MIM:610069]. Hereditary mixed polyposis syndrome (HMPS) is characterized by atypical juvenile polyps, colonic adenomas, and colorectal carcinomas.
Note=A microdeletion of chromosome 10q23 involving BMPR1A and PTEN is a cause of chromosome 10q23 deletion syndrome, which shows overlapping features of the following three disorders: Bannayan-Zonana syndrome, Cowden disease and juvenile polyposis syndrome. - Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain. - Cellular localizationMembrane.
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Database links
- Entrez Gene: 657 Human
- Entrez Gene: 657 Human
- Omim: 601299 Human
- SwissProt: P36894 Human
- SwissProt: P36894 Human
- Unigene: 524477 Human
Target information above from: UniProt accession
P36894
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 10q23del antibodyActivin A receptor type II like kinase 3 antibodyActivin receptor like kinase 3 antibody
- Activin receptor-like kinase 3 antibodyACVRLK 3 antibodyACVRLK3 antibodyALK 3 antibodyALK-3 antibodyALK3 antibodyBMP type-1A receptor antibodyBMPR 1A antibodyBmpr antibodyBMPR-1A antibodyBmpr1a antibodyBMR1A_HUMAN antibodyBone morphogenetic protein receptor type IA antibodyBone morphogenetic protein receptor type IA precursor antibodyBone morphogenetic protein receptor type-1A antibodyBR 1a antibodyBR1a antibodyCD 292 antibodyCD292 antibodyCD292 antigen antibodyEC 2.7.11.30 antibodySerine threonine protein kinase receptor R5 antibodySerine threonine protein kinase receptor R5 precursor antibodySerine/threonine-protein kinase receptor R5 antibodySKR 5 antibodySKR5 antibodyzBMPR IA antibodyzBMPRIA antibody
see all
Anti-BMPR1A antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BMPR1A antibody (ab59945)ab59945 at 1/50 - 1/100 dilution, staining BMPR1A in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining. -
Western blot - BMPR1A antibody (ab59945)Anti-BMPR1A antibody (ab59945) at 1 µg/ml +Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 60 kDa
Observed band size : 60 kDa -
Western blot - Anti-BMPR1A antibody (ab59945)Anti-BMPR1A antibody (ab59945) at 1/1000 dilution + Cultured Human lymphoblast whole cell lysate at 40 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 60 kDa
Observed band size : 60 kDa
Exposure time : 2 minutesThis image is courtesy of an anonymous Abreview
References for Anti-BMPR1A antibody (ab59945)
This product has been referenced in:
- Sinclair DC et al. Leiomyoma Simultaneously Impair Endometrial BMP-2-Mediated Decidualization and Anticoagulant Expression through Secretion of TGF-{beta}3. J Clin Endocrinol Metab 96:412-21 (2011). IHC-P ; Human . Read more (PubMed: 21084396) »
