Overview

  • Product nameAnti-BMPR1A antibodySee all BMPR1A primary antibodies ...
  • Description
    Rabbit polyclonal to BMPR1A
  • Tested applicationsWB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (10-30 aa in length) from the N-terminus of human BMPR1A, conjugated to KLH.

  • Positive control
    • Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab59945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • FunctionOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP-2 and BMP-4.
  • Tissue specificityHighly expressed in skeletal muscle.
  • Involvement in diseaseDefects in BMPR1A are a cause of juvenile polyposis syndrome (JPS) [MIM:174900]; also known as juvenile intestinal polyposis (JIP). JPS is an autosomal dominant gastrointestinal hamartomatous polyposis syndrome in which patients are at risk for developing gastrointestinal cancers. The lesions are typified by a smooth histological appearance, predominant stroma, cystic spaces and lack of a smooth muscle core. Multiple juvenile polyps usually occur in a number of Mendelian disorders. Sometimes, these polyps occur without associated features as in JPS; here, polyps tend to occur in the large bowel and are associated with an increased risk of colon and other gastrointestinal cancers.
    Defects in BMPR1A are a cause of Cowden disease (CD) [MIM:158350]. CD is an autosomal dominant cancer syndrome characterized by multiple hamartomas and by a high risk for breast, thyroid and endometrial cancers.
    Defects in BMPR1A are the cause of hereditary mixed polyposis syndrome 2 (HMPS2) [MIM:610069]. Hereditary mixed polyposis syndrome (HMPS) is characterized by atypical juvenile polyps, colonic adenomas, and colorectal carcinomas.
    Note=A microdeletion of chromosome 10q23 involving BMPR1A and PTEN is a cause of chromosome 10q23 deletion syndrome, which shows overlapping features of the following three disorders: Bannayan-Zonana syndrome, Cowden disease and juvenile polyposis syndrome.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 GS domain.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 10q23del antibody
    • Activin A receptor type II like kinase 3 antibody
    • Activin receptor like kinase 3 antibody
    • Activin receptor-like kinase 3 antibody
    • ACVRLK 3 antibody
    • ACVRLK3 antibody
    • ALK 3 antibody
    • ALK-3 antibody
    • ALK3 antibody
    • BMP type-1A receptor antibody
    • BMPR 1A antibody
    • Bmpr antibody
    • BMPR-1A antibody
    • Bmpr1a antibody
    • BMR1A_HUMAN antibody
    • Bone morphogenetic protein receptor type IA antibody
    • Bone morphogenetic protein receptor type IA precursor antibody
    • Bone morphogenetic protein receptor type-1A antibody
    • BR 1a antibody
    • BR1a antibody
    • CD 292 antibody
    • CD292 antibody
    • CD292 antigen antibody
    • EC 2.7.11.30 antibody
    • Serine threonine protein kinase receptor R5 antibody
    • Serine threonine protein kinase receptor R5 precursor antibody
    • Serine/threonine-protein kinase receptor R5 antibody
    • SKR 5 antibody
    • SKR5 antibody
    • zBMPR IA antibody
    • zBMPRIA antibody
    see all

Anti-BMPR1A antibody images

  • ab59945 at 1/50 - 1/100 dilution, staining BMPR1A in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.
  • Anti-BMPR1A antibody (ab59945) at 1 µg/ml + Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • Anti-BMPR1A antibody (ab59945) at 1/1000 dilution + Cultured Human lymphoblast whole cell lysate at 40 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-BMPR1A antibody (ab59945)

This product has been referenced in:
  • Sinclair DC  et al. Leiomyoma Simultaneously Impair Endometrial BMP-2-Mediated Decidualization and Anticoagulant Expression through Secretion of TGF-{beta}3. J Clin Endocrinol Metab 96:412-21 (2011). IHC-P ; Human . Read more (PubMed: 21084396) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Cultured Human Lymphoblasts)
Loading amount 40 µg
Specification Cultured Human Lymphoblasts
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"